Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
RANDOX LABORATORIES

Events

06 Jun 2016 - 09 Jun 2016
22 Jun 2016 - 24 Jun 2016
04 Jul 2016 - 06 Jul 2016

Novel Filtration System Removes Proteins and Phospholipids from Analytical Samples

By BiotechDaily International staff writers
Posted on 03 Feb 2014
Print article
Image: The ISOLUTE PLD+ protein and phospholipid removal plate (Photo courtesy of Biotage).
Image: The ISOLUTE PLD+ protein and phospholipid removal plate (Photo courtesy of Biotage).
Biotechnology and other life science researchers now have available a novel filter system that removes both proteins and phospholipids, which leaves samples ready for LC-MS/MS (liquid chromatography-mass spectroscopy) analysis.

The Biotage (Uppsala, Sweden) ISOLUTE PLD+ plate combines protein and phospholipid removal from blood-based matrix samples and provides simple and effective cleanup for LC-MS/MS analysis. According to the manufacturer, ISOLUTE PLD+ plates remove more than 99% of plasma proteins and phospholipids, the main causes of ion suppression, leading to cleaner extracts and increased sensitivity, signal-to-noise, for a broad range of analytes.

ISOLUTE PLD+ plates are available in standard 96-well format and can handle sample volumes of 100 to 200 microliters. Operation of the ISOLUTE PLD+ plate includes a filtration step that can be accomplished with a 96-well compatible positive pressure manifold (such as the Biotage Pressure+ 96), a vacuum manifold (for example the Biotage VacMaster 96) and most automated liquid handling systems.

“Requiring next to no method development, ISOLUTE PLD+ can be integrated quickly and easily into routine workflow, increasing productivity and reducing instrument downtime,” said Paul Roberts, analytical product manager at Biotage.

Related Links:

Biotage



Print article

Channels

Genomics/Proteomics

view channel
Image: Follicular helper T-cells (TFH cells, shown in blue) play a crucial role in the maturation of antibody-producing B-cells (shown in green). Activated B-cells give rise germinal centers (shown in red), where mature B-cells proliferate and produce highly specific antibodies against pathogens. Top left: normal germinal center in a mouse tonsil. All others: Germinal centers fail to form when the interaction between ICOS and TBK1 is interrupted (Photo courtesy of Dr. Kok-Fai Kong, La Jolla Institute for Allergy and Immunology).

Molecular Pathway Controlling High-affinity Antibody Production Identified

A molecular pathway has been identified that controls formation of follicular helper T-cells (TFH cells) germinal centers and production of high-affinity antibodies through interaction with the inducible... Read more

Drug Discovery

view channel

Experimental Small-Molecule Anticancer Drug Blocks RAS-binding Domains

The experimental small-molecule anticancer drug rigosertib was shown to block tumor growth by acting as an RAS-mimetic and interacting with the RAS binding domains of RAF kinases, resulting in their inability to bind to RAS, which inhibited the RAS-RAF-MEK pathway. Oncogenic activation of RAS genes due to point mutations... Read more

Biochemistry

view channel
Image: A space-filling model of the anticonvulsant drug carbamazepine (Photo courtesy of Wikimedia Commons).

Wastewater May Contaminate Crops with Potentially Dangerous Pharmaceuticals

Reclaimed wastewater used to irrigate crops is contaminated with pharmaceutical residues that can be detected in the urine of those who consumed such produce. Investigators at the Hebrew University... Read more

Business

view channel

European Biotech Agreement to Promote Antigen-Drug Conjugation Technology

Two European biotech companies have joined forces to exploit and commercialize an innovative, site-specific ADC (antigen-drug conjugate) conjugation technology. ProBioGen (Berlin, Germany), a company specializing in the development and manufacture of complex glycoproteins and Eucodis Bioscience (Vienna, Austria), a... Read more
Copyright © 2000-2016 Globetech Media. All rights reserved.