Features | Partner Sites | Information | LinkXpress
Sign In
GLOBETECH PUBLISHING LLC
GLOBETECH MEDIA
GLOBETECH PUBLISHING LLC

MicroRNA Studies to Be Enhanced by a New Line of Anti-miRNA Tools

By BiotechDaily International staff writers
Posted on 13 Feb 2014
Image: An artist's rendition of a Lentivirus expression vehicle (Photo courtesy of AMS Biotechnology ).
Image: An artist's rendition of a Lentivirus expression vehicle (Photo courtesy of AMS Biotechnology ).
A novel line of ready-to-use lentiviral expression vehicles provides microRNAs (miRNAs) and miRNA inhibitors for use as tools for the study of miRNAs and their role in various disease processes.

Lentiviruses, which are members of a subfamily of the retrovirus family, can deliver significant amounts of genetic information into host cells and integrate it into the cellular genome. Genetically engineered lentiviruses are therefore used as one of the most efficient tools of gene delivery. These modified lentiviruses contain a viral promoter that is used to control the expression of a transgene or shRNA but no residual virulence genes. Together with several other security modifications, this ensures their safe use in the laboratory.

MiRNAs comprise a family of small noncoding 19- to 25-nucleotide RNAs that regulate gene expression by targeting mRNAs in a sequence specific manner, inducing translational repression or mRNA degradation, depending on the degree of complementarity between miRNAs and their targets. Many miRNAs are conserved in sequence between distantly related organisms, suggesting that these molecules participate in essential processes. In fact, miRNAs have been shown to be involved in the regulation of gene expression during development, cell proliferation, apoptosis, glucose metabolism, stress resistance, and cancer.

Anti-miRNAs are miRNA inhibitors that are short RNA molecules complementary to the mature miRNA sequence. Anti-miRNA binds to endogenous miRNA and inhibits or down-regulates specific miRNAs. MiRNA inhibitors also show potential as therapeutic agents for some biological malfunctions.

AMS Biotechnology (Abingdon, United Kingdom) has introduced a range of ready-to-use human and mouse Lentivirus products for miRNA and anti-miRNA expression. This range of miRNA inhibitors was produced from optimally designed anti-miRNA lentivectors. AMS Biotechnology's anti-miRNA lentivectors were carefully designed for highest anti-miRNA expression levels with precise promoter transcription position, and to favor the antisense strand for RNA-induced silencing complex (RISC) processing.

Related Links:

AMS Biotechnology



Channels

Drug Discovery

view channel
Image: Molecular model of the protein Saposin C (Photo courtesy of Wikimedia Commons).

Nanovesicles Kill Human Lung Cancer Cells in Culture and in a Mouse Xenograft Model

Nanovesicles assembled from the protein Saposin C (SapC) and the phospholipid dioleoylphosphatidylserine (DOPS) were shown to be potent inhibitors of lung cancer cells in culture and in a mouse xenograft model.... Read more

Biochemistry

view channel

Possible New Target Found for Treating Brain Inflammation

Scientists have identified an enzyme that produces a class of inflammatory lipid molecules in the brain. Abnormally high levels of these molecules appear to cause a rare inherited eurodegenerative disorder, and that disorder now may be treatable if researchers can develop suitable drug candidates that suppress this enzyme.... Read more

Lab Technologies

view channel
Image: The FLUOVIEW FVMPE-RS Gantry microscope (Photo courtesy of Olympus).

New Multiphoton Laser Scanning Microscope Configurations Expand Research Potential

Two new configurations of a state-of-the-art multiphoton laser scanning microscope extend the usefulness of the instrument for examining rapidly occurring biological events and for obtaining images from... Read more

Business

view channel

Roche Acquires Signature Diagnostics to Advance Translational Research

Roche (Basel, Switzerland) will advance translational research for next generation sequencing (NGS) diagnostics by leveraging the unique expertise of Signature Diagnostics AG (Potsdam, Germany) in biobanks and development of novel NGS diagnostic assays. Signature Diagnostics is a privately held translational oncology... Read more
 
Copyright © 2000-2015 Globetech Media. All rights reserved.