Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
RANDOX LABORATORIES

Events

10 May 2016 - 16 May 2016
11 May 2016 - 13 May 2016

Molecular Pair Identified as Key to Host-Cell Invasion by Tick-Transmitted Pathogen

By BiotechDaily International staff writers
Posted on 22 Oct 2012
Print article
Image: Two Anaplasma phagocytophilum bacteria (arrows) bound to host cell surfaces; the bacterium on the right (thick arrow) is invading upon triggering its own uptake. (Photo courtesy of Jason Carlyon, PhD, and Matthew J. Troese, PhD / VCU.)
Image: Two Anaplasma phagocytophilum bacteria (arrows) bound to host cell surfaces; the bacterium on the right (thick arrow) is invading upon triggering its own uptake. (Photo courtesy of Jason Carlyon, PhD, and Matthew J. Troese, PhD / VCU.)
A pathogen-host “key and door” molecular pair has been identified as critical for the cell-cell adhesion required for invasion by the tick-transmitted pathogen Anaplasma phagocytophilum. The finding provides an important candidate target for development of drugs and a single vaccine against diseases caused by this and closely related pathogens.

A. phagocytophilum is an Anaplasmataceae family bacterium responsible for causing granulocytic anaplasmosis in humans; other tick-transmitted Anaplasmataceae species often cause diseases in domestic and livestock animals, including cattle. From studies in vitro using mammalian cell cultures and in vivo using mice, scientists at the Virginia Commonwealth University (VCU; Richmond, VA, USA) School of Medicine have shown that “outer membrane protein A” (OmpA) on the surface of A. phagocytophilum is critically important for its invasion of host cells. They also delineated a specific region in OmpA that mediates infection and is conserved among Anaplasmataceae family species. The research team showed that OmpA binds mammalian host cell surface sialylated glycoproteins and confirmed that this interaction is important for the adhesion stage of infection. "In other words, we identified both a key and door that together promote Anaplasma phagocytophilum infection," said lead investigator Jason A. Carlyon, PhD, associate professor, and a George and Lavinia Blick Scholar in the Department of Microbiology and Immunology of the VCU School of Medicine. "These findings are important because our data also establish a direction for development of a single vaccine that protects against members of an entire family of bacteria that cause disease," he added.

The study published online and planned to appear in print in the November 2012 issue of the journal Infection and Immunity (volume 80, issue 11), identifies the first A. phagocytophilum adhesin-receptor pair, and delineates the region of OmpA that is critical for infection. Researchers in Prof. Carlyon's laboratory are presently refining their understanding of the molecular basis for how OmpA promotes infection and are testing its efficacy in protecting against infection by A. phagocytophilum and other pathogenenic Anaplasmataceae members.

Related Links:
Virginia Commonwealth University



Print article

Channels

Genomics/Proteomics

view channel
Image: An expression of NOTCH 1 (green color) in ACC stem cells (Photo courtesy of Yale University).

Adenoid Cystic Carcinoma Stem Cells Depend on NOTCH1 and SOX10 Signaling

Cancer researchers have isolated a stem cell population from the cells making up an adenoid cystic carcinoma (ACC) tumor and showed that the signaling factors NOTCH1 and SOX10 were essential for the cancer... Read more

Drug Discovery

view channel

Experimental Small-Molecule Anticancer Drug Blocks RAS-binding Domains

The experimental small-molecule anticancer drug rigosertib was shown to block tumor growth by acting as an RAS-mimetic and interacting with the RAS binding domains of RAF kinases, resulting in their inability to bind to RAS, which inhibited the RAS-RAF-MEK pathway. Oncogenic activation of RAS genes due to point mutations... Read more

Lab Technologies

view channel

Huge Modifiable Biomedical Database to Be Available on the Wikidata Site

Genome researchers are exploiting the power of the open Internet community Wikipedia database to create a comprehensive resource for geneticists, molecular biologists, and other interested life scientists. While efficiency in generating scientific data improves almost daily, applying meaningful relationships between... Read more

Business

view channel

European Biotech Agreement to Promote Antigen-Drug Conjugation Technology

Two European biotech companies have joined forces to exploit and commercialize an innovative, site-specific ADC (antigen-drug conjugate) conjugation technology. ProBioGen (Berlin, Germany), a company specializing in the development and manufacture of complex glycoproteins and Eucodis Bioscience (Vienna, Austria), a... Read more
Copyright © 2000-2016 Globetech Media. All rights reserved.