We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
PURITAN MEDICAL

Download Mobile App




New Kits Designed for ELISA Assay Development

By LabMedica International staff writers
Posted on 23 May 2017
Print article
Image: The FlexLISA kit provides a flexible tool for development and optimization of sandwich ELISA assays (Photo courtesy of Innova Biosciences).
Image: The FlexLISA kit provides a flexible tool for development and optimization of sandwich ELISA assays (Photo courtesy of Innova Biosciences).
A novel line of kits for the development and optimization of sandwich ELISA assays is now available for biotech and other life science researchers.

The new Innova Biosciences FlexLISA kits are designed for the development and optimization of sandwich ELISA assays to detect the presence of any antigen with a high affinity for any antibody pair in complex samples (such as serum, plasma, or urine).

At the core of the kit is the 30 seconds hands-on time proprietary Lightning-Link conjugation technology for the labeling of the capture and detection antibodies of choice, and a biotin pre-coated 96 well strip-well plate provided either clear or black, depending on the enzymatic substrate.

The kit operates on a rapid one-step protocol that employs pre-blocked microtiter well strips coated with biotin. The FlexLISA assay is run by adding the sample and the antibody mix to the wells, incubating for one hour, washing, and reading the assay plate. FlexLISA is ideal for quick and reliable ELISA assay development, antibody pair screening, and ELISA assay optimization.

The main advantage of this format is that the amount of capture antibody is reduced up to 40-fold, allowing ELISAs to be developed in a cost-effective way using small amounts of commercially available antibodies. The format also allows more than one capture reagent to be used, allowing multiple analytes to be assessed on a single plate.

Anna Sereni, head of research and development at Innova Biosciences, said, “The FlexLISA kit provides researchers with optimum flexibility, and is ideal for quick and reliable ELISA assay development. This is a valuable addition to Innova’s product offering.”

Platinum Member
COVID-19 Rapid Test
OSOM COVID-19 Antigen Rapid Test
Specimen Collection & Transport
POCT Fluorescent Immunoassay Analyzer
FIA Go
New
Gold Member
Systemic Autoimmune Testing Assay
BioPlex 2200 ANA Screen with MDSS

Print article

Channels

Clinical Chemistry

view channel
Image: Reaching speeds up to 6,000 RPM, this centrifuge forms the basis for a new type of inexpensive, POC biomedical test (Photo courtesy of Duke University)

POC Biomedical Test Spins Water Droplet Using Sound Waves for Cancer Detection

Exosomes, tiny cellular bioparticles carrying a specific set of proteins, lipids, and genetic materials, play a crucial role in cell communication and hold promise for non-invasive diagnostics.... Read more

Molecular Diagnostics

view channel
Image: The study showed the blood-based cancer screening test detects 83% of people with colorectal cancer with specificity of 90% (Photo courtesy of Guardant Health)

Blood Test Shows 83% Accuracy for Detecting Colorectal Cancer

Colorectal cancer is the second biggest cause of cancer deaths among adults in the U.S., with forecasts suggesting 53,010 people might die from it in 2024. While fewer older adults are dying from this... Read more

Hematology

view channel
Image: The Gazelle Hb Variant Test (Photo courtesy of Hemex Health)

First Affordable and Rapid Test for Beta Thalassemia Demonstrates 99% Diagnostic Accuracy

Hemoglobin disorders rank as some of the most prevalent monogenic diseases globally. Among various hemoglobin disorders, beta thalassemia, a hereditary blood disorder, affects about 1.5% of the world's... Read more

Microbiology

view channel
Image: The new platform is designed to perform blood-based diagnoses of nontuberculosis mycobacteria (Photo courtesy of 123RF)

New Blood Test Cuts Diagnosis Time for Nontuberculous Mycobacteria Infections from Months to Hours

Breathing in nontuberculous mycobacteria (NTM) is a common experience for many people. These bacteria are present in water systems, soil, and dust all over the world and usually don't cause any problems.... Read more

Industry

view channel
Image: These new assays are being developed for use on the recently introduced DxI 9000 Immunoassay Analyzer (Photo courtesy of Beckman Coulter)

Beckman Coulter and Fujirebio Expand Partnership on Neurodegenerative Disease Diagnostics

Beckman Coulter Diagnostics (Brea, CA, USA) and Fujirebio Diagnostics (Tokyo, Japan) have expanded their partnership focused on the development, manufacturing and clinical adoption of neurodegenerative... Read more
Copyright © 2000-2024 Globetech Media. All rights reserved.