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New Kits Designed for ELISA Assay Development

By BiotechDaily International staff writers
Posted on 23 May 2017
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Image: The FlexLISA kit provides a flexible tool for development and optimization of sandwich ELISA assays (Photo courtesy of Innova Biosciences).
Image: The FlexLISA kit provides a flexible tool for development and optimization of sandwich ELISA assays (Photo courtesy of Innova Biosciences).
A novel line of kits for the development and optimization of sandwich ELISA assays is now available for biotech and other life science researchers.

The new Innova Biosciences FlexLISA kits are designed for the development and optimization of sandwich ELISA assays to detect the presence of any antigen with a high affinity for any antibody pair in complex samples (such as serum, plasma, or urine).

At the core of the kit is the 30 seconds hands-on time proprietary Lightning-Link conjugation technology for the labeling of the capture and detection antibodies of choice, and a biotin pre-coated 96 well strip-well plate provided either clear or black, depending on the enzymatic substrate.

The kit operates on a rapid one-step protocol that employs pre-blocked microtiter well strips coated with biotin. The FlexLISA assay is run by adding the sample and the antibody mix to the wells, incubating for one hour, washing, and reading the assay plate. FlexLISA is ideal for quick and reliable ELISA assay development, antibody pair screening, and ELISA assay optimization.

The main advantage of this format is that the amount of capture antibody is reduced up to 40-fold, allowing ELISAs to be developed in a cost-effective way using small amounts of commercially available antibodies. The format also allows more than one capture reagent to be used, allowing multiple analytes to be assessed on a single plate.

Anna Sereni, head of research and development at Innova Biosciences, said, “The FlexLISA kit provides researchers with optimum flexibility, and is ideal for quick and reliable ELISA assay development. This is a valuable addition to Innova’s product offering.”


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