We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
LGC Clinical Diagnostics

Download Mobile App




New Probes Promise Exceptional Live Cell Images

By LabMedica International staff writers
Posted on 09 May 2017
Print article
Image: An image generated by cancerous cells labeled with the new chemiluminescent probe (Photo courtesy of Dr. Doron Shabat, Tel Aviv University).
Image: An image generated by cancerous cells labeled with the new chemiluminescent probe (Photo courtesy of Dr. Doron Shabat, Tel Aviv University).
Development of a new generation of chemiluminescent molecules is expected to lead to synthesis of efficient chemiluminescent probes for microscopy that will be suitable for use under physiological conditions and should provide exceptional live cell images.

Chemiluminescent probes are considered to be among the most sensitive diagnostic tools that provide high signal-to-noise ratio for various applications such as DNA detection and immunoassays.

In this regard, investigators at Tel Aviv University have developed a new molecular methodology to design and foresee light-emission properties of turn-ON chemiluminescent dioxetane probes suitable for use under physiological conditions. The methodology is based on incorporation of a substituent on the benzoate species obtained during the chemiexcitation pathway of a Schaap’s adamantylidene–dioxetane probe. A striking substituent effect on the chemiluminescent efficiency of the probes was obtained when acrylate and acrylonitrile electron-withdrawing groups were installed. The chemiluminescent quantum yield of the best probe was more than three orders of magnitude higher than that of a standard, commercially available adamantylidene–dioxetane probe. These may be the most powerful chemiluminescent dioxetane probes synthesized to date that are suitable for use under aqueous conditions.

The investigators reported in the March 8, 2017, online edition of the journal ACS Central Science that one of the probes was capable of providing high-quality chemiluminescent cell images based on the endogenous activity of the enzyme beta-galactosidase. They suggested that this may have been the first demonstration of cell imaging achieved by a non-luciferin small-molecule probe with a direct chemiluminescent mode of emission.

"Chemiluminescence is considered one of the most sensitive methods used in diagnostic testing," said senior author Dr. Doron Shabat, professor of chemistry at Tel Aviv University. "We have developed a method to prepare highly efficient compounds that emit light upon contact with a specific protein or chemical. These compounds can be used as molecular probes to detect cancerous cells, among other applications."

Platinum Member
COVID-19 Rapid Test
OSOM COVID-19 Antigen Rapid Test
HLX
Complement 3 (C3) Test
GPP-100 C3 Kit
New
Gold Member
Fully Automated Cell Density/Viability Analyzer
BioProfile FAST CDV

Print article

Channels

Clinical Chemistry

view channel
Image: Reaching speeds up to 6,000 RPM, this centrifuge forms the basis for a new type of inexpensive, POC biomedical test (Photo courtesy of Duke University)

POC Biomedical Test Spins Water Droplet Using Sound Waves for Cancer Detection

Exosomes, tiny cellular bioparticles carrying a specific set of proteins, lipids, and genetic materials, play a crucial role in cell communication and hold promise for non-invasive diagnostics.... Read more

Molecular Diagnostics

view channel
Image: The study showed the blood-based cancer screening test detects 83% of people with colorectal cancer with specificity of 90% (Photo courtesy of Guardant Health)

Blood Test Shows 83% Accuracy for Detecting Colorectal Cancer

Colorectal cancer is the second biggest cause of cancer deaths among adults in the U.S., with forecasts suggesting 53,010 people might die from it in 2024. While fewer older adults are dying from this... Read more

Hematology

view channel
Image: The Gazelle Hb Variant Test (Photo courtesy of Hemex Health)

First Affordable and Rapid Test for Beta Thalassemia Demonstrates 99% Diagnostic Accuracy

Hemoglobin disorders rank as some of the most prevalent monogenic diseases globally. Among various hemoglobin disorders, beta thalassemia, a hereditary blood disorder, affects about 1.5% of the world's... Read more

Microbiology

view channel
Image: The new platform is designed to perform blood-based diagnoses of nontuberculosis mycobacteria (Photo courtesy of 123RF)

New Blood Test Cuts Diagnosis Time for Nontuberculous Mycobacteria Infections from Months to Hours

Breathing in nontuberculous mycobacteria (NTM) is a common experience for many people. These bacteria are present in water systems, soil, and dust all over the world and usually don't cause any problems.... Read more

Industry

view channel
Image: These new assays are being developed for use on the recently introduced DxI 9000 Immunoassay Analyzer (Photo courtesy of Beckman Coulter)

Beckman Coulter and Fujirebio Expand Partnership on Neurodegenerative Disease Diagnostics

Beckman Coulter Diagnostics (Brea, CA, USA) and Fujirebio Diagnostics (Tokyo, Japan) have expanded their partnership focused on the development, manufacturing and clinical adoption of neurodegenerative... Read more
Copyright © 2000-2024 Globetech Media. All rights reserved.