Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
RANDOX LABORATORIES

MicroRNA Protects Normal Brain from Trauma and Stress

By BiotechDaily International staff writers
Posted on 14 Jun 2017
Print article
Image: Research has identified a microRNA that can protect the brain from epilepsy-related trauma and stress (Photo courtesy of Getty Images).
Image: Research has identified a microRNA that can protect the brain from epilepsy-related trauma and stress (Photo courtesy of Getty Images).
A microRNA has been identified that helps prevent the normal brain from becoming susceptible to the type of seizures induced by trauma or stress in individuals with epilepsy.

MicroRNAs (miRNAs) are a small noncoding family of 19- to 25-nucleotide RNAs that regulate gene expression by targeting messenger RNAs (mRNAs) in a sequence specific manner, inducing translational repression or mRNA degradation, depending on the degree of complementarity between miRNAs and their targets. Many miRNAs are conserved in sequence between distantly related organisms, suggesting that these molecules participate in essential processes. In fact, miRNAs have been shown to be involved in the regulation of gene expression during development, cell proliferation, apoptosis, glucose metabolism, stress resistance, and cancer.

Epilepsy may have genetic or pharmacological origins or may develop following injury. However, it remains unclear how the normal brain escapes susceptibility to seizures.

Investigators at the Hebrew University of Jerusalem (Israel) hypothesized that rapidly inducible microRNAs could mediate such protection. To test this theory, they combined genetic engineering of mouse lines, RNA sequencing, electric recording of brain activity, and learning tests. Specifically, they engineered dTg-211 mice to overproduce a doxycycline-suppressible form of the microRNA miR-211 in their forebrains.

Results published in the June 5, 2017, online edition of the journal Proceedings of the [U.S.] National Academy of Sciences revealed that the genetically engineered mice reacted to doxycycline exposure - which lowered levels of miR-211 - by spontaneous electrocorticography-documented nonconvulsive seizures, accompanied by forebrain accumulation of the convulsive seizures mediating microRNA, miR-134. RNA sequencing of the doxycycline-treated dTg-211 mice showed overrepresentation of synaptic activity, Ca2+ transmembrane transport, TGFBR2 (transforming growth factor, beta receptor II) signaling, and cholinergic synapse pathways.

"Dynamic changes in the amount of miR-211 in the forebrains of these mice shifted the threshold for spontaneous and pharmacologically induced seizures, alongside changes in the cholinergic pathway genes," said senior author Dr. Hermona Soreq, professor of molecular neuroscience at the Hebrew University of Jerusalem. "It is important to discover how only some people's brains present a susceptibility to seizures, while others do not, even when subjected to these same stressors. In searching for the physiological mechanisms that allow some people's brains to avoid epilepsy, we found that increased levels of micro-RNA 211 could have a protective effect."

Related Links:
Hebrew University of Jerusalem


Print article

Channels

Biochemistry

view channel
Image: A space-filling model of the anticonvulsant drug carbamazepine (Photo courtesy of Wikimedia Commons).

Wastewater May Contaminate Crops with Potentially Dangerous Pharmaceuticals

Reclaimed wastewater used to irrigate crops is contaminated with pharmaceutical residues that can be detected in the urine of those who consumed such produce. Investigators at the Hebrew University... Read more

Business

view channel

Collaborative Agreement to Aid in Setting Guidelines for Evaluating Potential Ebola Therapy

Cooperation between an Israeli biopharmaceutical company and medical branches of the US government is designed to set ground rules for continued evaluation of an experimental therapy for Ebola virus disease. RedHill Biopharma Ltd. (Tel Aviv, Israel), a biopharmaceutical company primarily focused on development and c... Read more
Copyright © 2000-2017 Globetech Media. All rights reserved.