New Multimode Microplate Reader Delivers High Resolution Performance

A newly available next generation microplate reader performs luminescence, fluorescence, and absorbance measurements with the sensitivity and resolution usually obtained only from separate, dedicated instruments.

The Berthold Technologies (Bad Wildbad, Germany) Mithras2 LB943 microplate reader is a high-end multimode reader based on monochromator technology. The instrument is equipped with two double monochromators with high blocking efficiency and high f-number (increased transmission) for filter free absorbance and fluorescence measurements.

The Mithras2 was designed to carry out an impressive variety of assays including Luminescence, BRET (Bioluminescence Resonance Energy Transfer), fluorescence, FRET (Fluorescence Resonance Energy Transfer), fluorescence polarization (FP), UV/VIS absorbance, time-resolved fluorescence, and time-resolved-FRET. To accomplish these varied tasks the instrument was designed with a proprietary optical system consisting of separate paths for different reading technologies, thus offering quality analysis usually obtained only from separate dedicated instruments.

The instrument can be configured for up to four reagent injectors programmed to dispense multiple activators or detection reagents with user-adjustable volumes at any time during the measurement process. The injectors are based on proprietary JET injection technology that delivers superior accuracy and precision. Variable injections speeds and "bio-friendly" materials enable injection of live cells.

The Mitras2 can be used to measure enzyme activities, phagocytosis, calcium flux, cell viability, apoptosis, immunoassays, protein and nucleic acid concentrations, and protein-protein interactions. Single and multiple endpoint measurements as well as kinetic and scanning measurements can be performed. The data can be displayed numerically and graphically and may be printed or exported to a spreadsheet.

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