Features | Partner Sites | Information | LinkXpress
Sign In
GLOBETECH MEDIA
GLOBETECH PUBLISHING LLC
GLOBETECH PUBLISHING LLC

Antibodies to Cytokine Protect Eyes During Bacterial Infection

By BiotechDaily International staff writers
Posted on 31 Oct 2012
Study shows improved pathology of bacterial eye infection upon topical treatment with polyclonal antibodies against the main neutrophil recruiting, proinflammatory cytokine; surprisingly, bacterial clearance was also improved.

Severe tissue damage during bacterial infections is often largely caused by inflammatory immune system by-products, noxious substances released from neutrophils attracted to the infection site to clear away the pathogen. As a key to attracting neutrophils, the proinflammatory cytokine interleukin-17 (IL-17) contributes to both microbial clearance as well as to inflammation-associated tissue damage.

A study led by researchers at Brigham and Women’s Hospital (Boston, MA, USA) investigated in vivo roles of IL-17 in mammalian eye infections using Pseudomonas aeruginosa to cause ulcerative keratitis after scratch injury in a mouse model. Two experimental approaches were used: examination of IL-17 receptor (IL-17R) deficient mice and testing of a topical treatment with polyclonal antibodies against IL-17. Pseudomonas aeruginosa was chosen mainly due to it being a common cause of corneal infections (particularly in those at higher risk such as extended-wear contact lens users) and as it is often found to be resistant to antibiotics.

The findings, published in the journal Infection and Immunity, October 2012, from both approaches showed that neutralization of IL-17 during P. aeruginosa corneal infection reduces neutrophil influx and pathology without compromising bacterial clearance. The antibody-mediated IL-17 neutralization approach was tested with six different laboratory or clinical P. aeruginosa strains, including both invasive and cytotoxic strains, all of which gave essentially the same results.

The strategy of inhibiting neutrophil tissue infiltration risked reducing the immune system’s bacteria-killing function - “We thought that blocking IL-17 infection might worsen eye infections,” said principal investigator Prof. Gregory P. Priebe of Brigham and Women’s Hospital and Boston Children’s Hospital, “Surprisingly, just the opposite was seen: blocking IL-17 with antibodies led both to fewer neutrophils in the eye, and to fewer bacteria.” The results therefore offer a new avenue for therapy of these sight-threatening infections.

Related Links:
Brigham and Women’s Hospital


WATERS CORPORATION

Channels

Genomics/Proteomics

view channel
Image: Electron micrograph of Hepatitis C virus purified from cell culture. Scale bar is 50 nanometers (Photo courtesy of the Center for the Study of Hepatitis C, the Rockefeller University).

Oxidized LDL Predicts Response to Interferon Treatment of Chronic Hepatitis C and May Be a Treatment Option

Oxidized low-density lipoprotein (oxLDL) in the blood was shown to predict responsiveness to interferon treatment in patients with chronic Hepatitis C virus (HCV) infection and to inhibit spread of the... Read more

Drug Discovery

view channel
Image: Molecular model of the anti-cancer drug 5-fluorouracil (Photo courtesy of Wikimedia Commons).

Novel Microcapsule Approach Reduces Toxic Side Effects of Chemotherapy

Cancer researchers have reduced chemotherapy's toxic side effects by using nanoporous capsules to transport an enzyme to the site of a tumor where it is activated by a selective heating process to convert... Read more

Lab Technologies

view channel
Image: The gene assembly robot, the GeneTheatre (Photo courtesy of Analytik Jena AG).

Genomic Research Laboratories Await New Compact Liquid Handling System

A small footprint benchtop liquid handler that automates multiple gene assembly tasks and associated procedures such as PCR setup is now available for use by biotech and genomic research laboratories.... Read more

Business

view channel

NanoString and MD Anderson Collaborate on Development of Novel Multi-Omic Expression Profiling Assays for Cancer

The University of Texas MD Anderson Cancer Center (Houston, TX, USA) and NanoString Technologies, Inc. (Seattle, WA, USA) will partner on development of a revolutionary new type of assay—simultaneously profiling gene and protein expression, initially aiming to discover and validate biomarker signatures for immuno-oncology... Read more
 

Events

02 Jun 2015 - 03 Jun 2015
15 Jun 2015 - 18 Jun 2015
Copyright © 2000-2015 Globetech Media. All rights reserved.