Features | Partner Sites | Information | LinkXpress
Sign In
GLOBETECH PUBLISHING LLC
PZ HTL SA
GLOBETECH PUBLISHING LLC

New Molecular Biology Tool Eases Preparation of Chromatin-DNA Complexes

By BiotechDaily International staff writers
Posted on 21 Jul 2014
Image
Image
Biotech and other life sciences laboratories that isolate and analyze chromatin-DNA complexes will have a much easier time, as a new molecular biology tool for this purpose has now been released to the market.

The new Porvair Sciences Ltd. (Wales, United Kingdom) Chromatrap solid state ChIP (chromatin immunoprecipitation) assay is described as being more efficient, sensitive and robust than standard ChIP methods.

ChiP is a fast growing research technique and is commonly used for mapping the DNA-protein interactions in cells which are crucial for correct gene regulation. For example, they may be used to determine whether proteins such as transcription factors and modified histones bind to a particular region of DNA of living cells or tissues. In a ChIP assay, fragments of the DNA-protein complex (chromatins) are cross-linked in such a way so as to retain the specific DNA-protein interactions. The chromatin is then extracted and sheared either by sonication or enzymatic digestion into small fragments. The DNA/protein fragments are selectively immunoprecipitated using antibodies directed against the protein of interest and the resulting fractions treated to separate the DNA and protein components. Polymerase Chain Reaction (PCR), real time PCR, hybridization on microarrays, or direct sequencing are typically used to identify DNA fragments of defined sequence.

Chromatrap kits use revolutionary spin columns or microplates, which contain discs of an inert, porous polymer to which protein A or G has been covalently attached. During an assay, the chromatin/antibody complex is selectively retained by the disc. Flushing with three buffers and an elution step are all that is required to obtain the selectively enriched DNA. Chromatrap allows high quality chromatin to be obtained from as few as 7,000 cells in under five hours.

Chromatrap was developed by Porvair in association with Dr. Steve Conlan, professor of molecular and cell biology at Swansea University (United Kingdom).

Related Links:

Porvair Sciences Ltd.

Swansea University



SLAS - Society for Laboratory Automation and Screening
BIOSIGMA S.R.L.
RANDOX LABORATORIES
comments powered by Disqus

Channels

Drug Discovery

view channel

Omega 3 Found to Improve Behavior in Children with ADHD

Supplements of the fatty acids omega 3 and 6 can help children and adolescents who have a specific kind of have attention deficit hyperactivity disorder (ADHD). Moreover, these findings indicate that a customized cognitive training program can improve problem behavior in children with ADHD. Statistics show that 3%–6%... Read more

Biochemistry

view channel

Blocking Enzyme Switch Turns Off Tumor Growth in T-Cell Acute Lymphoblastic Leukemia

Researchers recently reported that blocking the action of an enzyme “switch” needed to activate tumor growth is emerging as a practical strategy for treating T-cell acute lymphoblastic leukemia. An estimated 25% of the 500 US adolescents and young adults diagnosed yearly with this aggressive disease fail to respond to... Read more

Business

view channel

Two Industry Partnerships Initiated to Fuel Neuroscience Research

Faster, more complex neural research is now attainable by combining technology from two research companies. Blackrock Microsystems, LLC (Salt Lake City, UT, USA), a developer of neuroscience research equipment, announced partnerships with two neuroscience research firms—PhenoSys, GmbH (Berlin, Germany) and NAN Instruments, Ltd.... Read more
 
Copyright © 2000-2014 Globetech Media. All rights reserved.