Features | Partner Sites | Information | LinkXpress
Sign In
GLOBETECH PUBLISHING LLC
GLOBETECH MEDIA
GLOBETECH PUBLISHING LLC

Image Analysis, Environmental Control and Experiment Handling via Mobile Devices for Life Science Research

By BiotechDaily International staff writers
Posted on 23 Jun 2014
Leica Application Suite Advanced Fluorescence (LAS AF)
Software platform (Photo courtesy of LEICA)
Using a novel software platform designed for advanced life-science research in wide-field and confocal microscopy, advanced fluorescence (AF) researchers can now perform two-dimensional (2D) image analysis on multichannel experiments and completely control the environmental conditions of an experiment. Furthermore, users of wide-field systems can now connect to the acquisition station remotely anywhere at any time.

Leica Microsystems (Wetzlar, Germany) has launched the Leica Application Suite Advanced fluorescence (LAS AF), software platform in its 4.0 version. A workflow guides users step by step through the 2D image analysis from applying filters, thresholding, and binary image processing, to measurements and classification. Findings are obtained simply, rapidly, and reproducibly, even with multidimensional data sets. Each analysis step offers a set of tools that researchers can choose according to the analysis requirements. The image viewer provides immediate feedback on the applied settings. Analysis workflows can be saved as user-defined protocols and applied to further data sets at a later time. The analysis results can be saved with the experiment or exported as Excel reports together with histograms and images of intermediate steps for documentation or further analysis. For statistical purposes users can analyze several data sets of experiments in one run by simply adding the data to a batch list.

Samples with several fluorescent markers can be completely analyzed in one run by using separate analysis channels for different markers. Optionally, objects identified in different acquisition channels can be correlated, for example to count the number of DNA strand breaks per nucleus. 2D analysis can also be applied to color images, i.e., to determine the number of healthy cells in one analysis channel versus the number of abnormal cells in another analysis channel.

Knowledge of all environmental parameters is vital for researchers. The correct environmental conditions help minimize experiment failures and enable researchers to fully assess their results. With the Environmental Control module in LAS AF 4.0 users can monitor temperature, CO2 and O2 conditions at all times. They can also predefine changes of these environmental parameters during an experiment and even let the system wait until a specified temperature is reached before starting. The range control either displays a warning if user-defined limits are exceeded or, alternatively, it stops the whole experiment. All environmental parameter controls are combined in one user-interface, making it easy to keep track. In combination with the LAS AF Live Data Mode module, acquisition macros can be defined to run temperature profiles – for example to perform heat shock experiments.

On wide-field systems, the LAS AF Mobile Connection module enables researchers to stay connected to and interact with their experiments 24 hours a day, seven days a week—without the need to stay close to the imaging system. Users can remotely start, stop, or pause their experiments, capture images to identify the best time to start an experiment or display an image at a conference that has been taken seconds ago during a running experiment.

Mobile Connection works via web client or mobile device. As several users can be connected with the microscope system at the same time, they can also interact with each other, exchanging messages to discuss the most recent developments of a live experiment.

Related Links:

Leica Microsystems




Read the full article by registering today, it's FREE! It's Free!
Register now for FREE to BiotechDaily.com and get complete access to news and events that shape the world of biotechnology.
Free digital version edition of Bio Research International sent by email on regular basis
Free print version of Bio Research International magazine
(available only outside USA and Canada).
Free and unlimited access to back issues of Bio Research International in digital format
Free Bio Research International Newsletter sent every 2 weeks containing the latest news
Free breaking news sent via email
Free access to Events Calendar
Free access to LinkXpress new product services
REGISTRATION IS FREE AND EASY!


Click here to Register

Sign in: Registered website members
Username: Password:
Forgot username/password? Click here!
Sign in: Registered magazine subscribers
Subcode: Last Name:
What is SUBCODE?


Channels

Genomics/Proteomics

view channel
Image: Cancer cells, left, were pretreated with a drug that blocks the ERK signal, and right, without the pretreatment. Top cells are untreated, while the bottom ones are stimulated (Photo courtesy of the Weizmann Institute of Science).

Prevention of ERK Nuclear Translocation Blocks Cancer Proliferation in Animal Models

A team of cell biologists has shown that the cancer promoting effects of ERK dysregulation can be blocked by low molecular weight drugs that prevent translocation of this kinase from the cells' cytoplasm... Read more

Drug Discovery

view channel
Image: Star-like glial cells in red surround alpha-beta plaques in the cortex of a mouse with a model of Alzheimer\'s disease (Photo courtesy of Strittmatter laboratory/Yale University).

Experimental Cancer Drug Reverses Symptoms in Mouse Model of Alzheimer's Disease

An experimental, but clinically disappointing drug for treatment of cancer has been found to be extremely effective in reversing the symptoms of Alzheimer's disease (AD) in a mouse model.... Read more

Biochemistry

view channel
Image:  Model depiction of a novel cellular mechanism by which regulation of cryptochromes Cry1 and Cry2 enables coordination of a protective transcriptional response to DNA damage caused by genotoxic stress (Photo courtesy of the journal eLife, March 2015, Papp SJ, Huber AL, et al.).

Two Proteins Critical for Circadian Cycles Protect Cells from Mutations

Scientists have discovered that two proteins critical for maintaining healthy day-night cycles also have an unexpected role in DNA repair and protecting cells against genetic mutations that could lead... Read more

Business

view channel

NanoString and MD Anderson Collaborate on Development of Novel Multi-Omic Expression Profiling Assays for Cancer

The University of Texas MD Anderson Cancer Center (Houston, TX, USA) and NanoString Technologies, Inc. (Seattle, WA, USA) will partner on development of a revolutionary new type of assay—simultaneously profiling gene and protein expression, initially aiming to discover and validate biomarker signatures for immuno-oncology... Read more
 

Events

21 Apr 2015 - 23 Apr 2015
21 Apr 2015 - 23 Apr 2015
Copyright © 2000-2015 Globetech Media. All rights reserved.