Features | Partner Sites | Information | LinkXpress
Sign In
GLOBETECH PUBLISHING
GLOBETECH PUBLISHING
JIB

Mass Spectrometry Technology Maps Chemicals as They Migrate Into Skin

By BiotechDaily International staff writers
Posted on 14 May 2014
A mass spectrometry technique gaining acceptance for medical applications such as imaging tumor surfaces can also be used to analyze the migration of small-molecule compounds applied to the skin. Because skin is such a complicated organ, the technology could be a helpful for developing transdermal drugs.

The study’s findings were published April 28, 2014, in the Journal of the American Chemical Society. Stanford University (Stanford, CA, USA) chemistry Profs. Richard N. Zare and Justin Du Bois, postdoc Livia S. Eberlin, graduate student John V. Mulcahy, and colleagues revealed that desorption electrospray ionization-mass spectrometry (DESI-MS) imaging has many advantages over other approaches that require complicated preparation of skin samples.

Moreover, DESI-MS imaging can be performed under ambient settings, instead of in a vacuum condition, as other MS methods require. Furthermore, test compounds do not have to be radioactively labeled or tagged with unwieldy dye molecules that could affect the compounds’ normal migration through skin. “That’s why this method is very appealing,” said Mark R. Prausnitz, a chemical and biomolecular engineering professor who heads the Laboratory for Drug Delivery at Georgia Institute of Technology (Atlanta, GA, USA).

DESI-MS was developed 10 years ago and involves spraying charged solvent droplets at a surface. Backsplash droplets containing dissolved molecules are then captured and examined using a mass spectrometer. The technology has been used for medical applications such as imaging drugs in tissue samples.

The Stanford scientists chose a number of small molecules that change sodium channels in skin cells, including lidocaine and a shellfish toxin. They applied them to the surface of skin samples and were able to track the compounds’ migration to a depth of 1.2 mm.

Such studies of drug migration are required to enlarge the limited selection of transdermal drugs, according to Prof. Prausnitz. Only approximately 30 agents, such as nicotine, have transdermal versions. The drugs must be small, lipophilic, and effective at a low dose. With this newly adapted tool, however, scientists could more readily study methods to enhance skin permeation, Prof. Prausnitz reported. “We’re very interested in the pathway--which part of the skin did the drug go through?”

Related Links:

Stanford University



comments powered by Disqus

Channels

Genomics/Proteomics

view channel
Image: A leukemia cell coated with antibody is marked for destruction by activated natural killer cells (Photo courtesy of the University of Southern California).

Leukemia Cells Are Killed in Culture by Immune Cells Grown from the Same Patient

Immune system natural killer (NK) cells were isolated from leukemia patients, expanded in culture, and then shown in an in vitro system to attack and destroy cancer cells from the original cell donors.... Read more

Drug Discovery

view channel
Image: Synthetic ion transporters can induce apoptosis by facilitating chloride anion transport into cells (Photo courtesy of the University of Texas, Austin).

Experimental Drug Kills Cancer Cells by Interfering with Their Ion Transport Mechanism

An experimental anticancer drug induces cells to enter a molecular pathway leading to apoptosis by skewing their ion transport systems to greatly favor the influx of chloride anions. To promote development... Read more

Therapeutics

view channel
Image: Liver cells regenerated in mice treated with a new drug (right) compared with a control group (center) after partial liver removal. Healthy liver cells are shown at left (Photo courtesy of Marshall et al, 2014, the Journal of Experimental Medicine).

New Drug Triggers Liver Regeneration After Surgery

Investigators have revealed that an innovative complement inhibitor decreases complement-mediated liver cell death, and actually stimulates postsurgery liver regrowth in mice. Liver cancer often results... Read more

Business

view channel

Partnership Established to Decode Bowel Disease

23andMe (Mountain View, CA,USA), a personal genetics company, is collaborating with Pfizer, Inc. (New York, NY, USA), in which the companies will seek to enroll 10,000 people with inflammatory bowel disease (IBD) in a research project designed to explore the genetic factors associated with the onset, progression, severity,... Read more
 
Copyright © 2000-2014 Globetech Media. All rights reserved.