Features | Partner Sites | Information | LinkXpress
Sign In
GLOBETECH PUBLISHING LLC
GLOBETECH PUBLISHING LLC
GLOBETECH MEDIA

Graphene Sandwich Technology Reveals Clues into Inner Workings of Proteins

By BiotechDaily International staff writers
Posted on 30 Dec 2013
Image: Artist impression of a graphene sheet (Photo courtesy of the University of Manchester).
Image: Artist impression of a graphene sheet (Photo courtesy of the University of Manchester).
British scientists have discovered that the most fragile, microscopic compounds can be saved from the harmful effects of radiation when under the microscope if they are “sandwiched” between two sheets of graphene. The technique could soon be the answer to being able to directly study every single individual atom in a protein chain—something that has not yet been achieved—and transform the determination of cell structure, how the immune system reacts to viruses, and help in the design of new antiviral drugs.

Seeing the structure of some the smallest of objects at the atomic level, such as proteins and other sensitive two-dimensional (2D) substances, requires a powerful electron microscope. This is extremely problematic because the radiation from the electron beam can kill the very fragile object being imaged before any valuable data can be effectively recorded. However, by protecting fragile objects between two sheets of graphene they can be imaged for longer without damage under the electron beam, making it possible to quantitatively identify every single atom within the structure.

This technique has been shown to be very successful on the test case of a fragile inorganic 2D crystal, and the study’s findings were published November 26, 2013, in the journal ACSNano. The research was conducted by investigators from the University of Manchester (UK) and the SuperSTEM facility, which is located at Science & Technology Facilities’ Council (STFC) Daresbury Laboratory (UK), and funded by the Engineering and Physical Sciences Research Council (EPSRC; Swindon, Wiltshire, UK).

The team of scientists, which included Sir Kostya Novoselov, who shared a Nobel Prize in Physics in 2010 for utilizing the remarkable characteristics of graphene, was able to visualize the effects of encasing a microscopic crystal of another highly delicate 2D material, molybdenum disulfide, between two sheets of graphene. They discovered that they were able to apply a high electron beam to directly image, identify, and obtain complete chemical analysis of each and every atom within the molybdenum di-sulfide sheet, without causing any defects to the compound through radiation.

The University of Manchester’s Dr. Recep Zan, who led the research team, said, “Graphene is a million times thinner than paper, yet stronger than steel, with fantastic potential in areas from electronics to energy. But this research shows its potential in biochemistry could also be just as significant, and could eventually open up all sorts of applications in the biotechnology arena.”

Prof. Quentin Ramasse, scientific director at SuperSTEM ,added, “What this research demonstrates is not so much about graphene itself, but how it can impact the detail and accuracy at which we can directly study other inorganic 2D materials or highly fragile molecules. Until now, this has mostly been possible through less direct and often complicated methods such as protein crystallography, which do not provide a direct visualization of the object in question. This new capability is particularly exciting, because it could pave the way to being able to image every single atom in a protein chain for example, something which could significantly impact our development of treatments for conditions such as cancer, Alzheimer’s and HIV.”

Related Links:

University of Manchester
Science & Technology Facilities’ Council Daresbury Laboratory



Channels

Genomics/Proteomics

view channel
Image: Many molecular biology studies begin with purified DNA and RNA extracted from complex environments such as the human gut (Photo courtesy of Los Alamos [US] National Laboratory).

New Metagenomics Analysis Tool Reduces False Discovery Rates

Genomic researchers recently described a novel new tool for analyzing the complex data generated during DNA screens of mixed populations of organisms such as the human gut microbiome. DNA screening... Read more

Drug Discovery

view channel
Image: Wafers like the one shown here are used to create “organ-on-a-chip” devices to model human tissue (Photo courtesy of Dr. Anurag Mathur, University of California, Berkeley).

Human Heart-on-a-Chip Cultures May Replace Animal Models for Drug Development and Safety Screening

Human heart cells growing in an easily monitored silicon chip culture system may one day replace animal-based model systems for drug development and safety screening. Drug discovery and development... Read more

Biochemistry

view channel
Image:  Model depiction of a novel cellular mechanism by which regulation of cryptochromes Cry1 and Cry2 enables coordination of a protective transcriptional response to DNA damage caused by genotoxic stress (Photo courtesy of the journal eLife, March 2015, Papp SJ, Huber AL, et al.).

Two Proteins Critical for Circadian Cycles Protect Cells from Mutations

Scientists have discovered that two proteins critical for maintaining healthy day-night cycles also have an unexpected role in DNA repair and protecting cells against genetic mutations that could lead... Read more

Business

view channel

“Softer” Mass Spec Techniques Gain Advantage in Biomarker Discovery

Two mass spectrometry (MS) technologies, MALDI and DESI, are increasing in applications as their effectiveness is established, according to Kalorama Information (New York, NY, USA) in its report “Proteomics Markets for Research and IVD Applications (Mass Spectrometry, Chromatography, Microarrays, Electrophoresis, Immunoassays,... Read more
 
Copyright © 2000-2015 Globetech Media. All rights reserved.