Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
RANDOX LABORATORIES

New Module Enables Super-Resolution Microscopy of Cell Organelles in Three Dimensions

By BiotechDaily International staff writers
Posted on 20 Nov 2013
Print article
Image: The ELYRA super-resolution microscope system enables 3D-PALM imagery (Photo courtesy of Zeiss).
Image: The ELYRA super-resolution microscope system enables 3D-PALM imagery (Photo courtesy of Zeiss).
Biotech researchers and other life scientists who employ advanced microscopy techniques are now able to move up to a state-of-the-art super-resolution microscopy system.

Zeiss (Jena, Germany) has introduced ELYRA P.1, a new module that makes possible three-dimensional (3D) super-resolution photoactivated localization microscopy (PALM) for endogenously-expressed photo-switchable fluorescent proteins.

In PALM, photo-switchable fluorescent molecules are sparsely activated so that only one out of many will be in its on-state within a single point spread function (PSF). In 3D, the PSF shape codes for the z-position. The localizations are plotted in a new image to create the super-resolved image. ELYRA P.1 achieves resolutions in the range of 20–30 nanometers laterally and 50–80 nanometers axially. The ELYRA P.1 module captures highly resolved structures in 3D, while treating the sample so gently that it remains available for long-term observation.

The ELYRA system can be integrated with the Zeiss laser scanning microscopes LSM 710 or LSM 780. In addition, ELYRA works seamlessly together with Zeiss scanning electron microscopes.

“With ELYRA, researchers can investigate the structural arrangement of one or multiple proteins, reveal the ultrastructure of cell organelles in 2D and 3D as well as map and count molecules within a structure. Sophisticated algorithms relate photon statistics to precision information in all directions, so researchers can display their structures fully rendered in 3D,” said Dr. Klaus Weisshart, ELYRA product manager at Zeiss.

Related Links:

Zeiss



Print article

Channels

Genomics/Proteomics

view channel
Image: The green-labeled cells show a basal cell carcinoma in mouse tail epidermis derived from a single mutant stem cell and expanding out of the normal epidermis stained in red (Photo courtesy of Adriana Sánchez-Danés, Université Libre de Bruxelles).

Stem Cells Not Progenitors Can Trigger Skin Cancer Growth

Cancer researchers have discovered that stem cells can initiate development of malignant skin tumors, while progenitor cells are limited to triggering only benign growths. A progenitor cell is similar... Read more

Biochemistry

view channel
Image: A space-filling model of the anticonvulsant drug carbamazepine (Photo courtesy of Wikimedia Commons).

Wastewater May Contaminate Crops with Potentially Dangerous Pharmaceuticals

Reclaimed wastewater used to irrigate crops is contaminated with pharmaceutical residues that can be detected in the urine of those who consumed such produce. Investigators at the Hebrew University... Read more

Business

view channel

Sartorius Acquires US Start-up ViroCyt

Sartorius AG (Göttingen, Germany), a pharmaceutical and laboratory equipment provider, has acquired ViroCyt Incorporated (Broomfield, CO, USA), a start-up in the field of rapid virus quantification, in a deal valued at approximately USD 16 million. ViroCyt’s automated platform integrates instruments, software and reagents... Read more
Copyright © 2000-2016 Globetech Media. All rights reserved.