Features | Partner Sites | Information | LinkXpress
Sign In
JIB
GLOBETECH PUBLISHING
BioConferenceLive

New Module Enables Super-Resolution Microscopy of Cell Organelles in Three Dimensions

By BiotechDaily International staff writers
Posted on 20 Nov 2013
Image: The ELYRA super-resolution microscope system enables 3D-PALM imagery (Photo courtesy of Zeiss).
Image: The ELYRA super-resolution microscope system enables 3D-PALM imagery (Photo courtesy of Zeiss).
Biotech researchers and other life scientists who employ advanced microscopy techniques are now able to move up to a state-of-the-art super-resolution microscopy system.

Zeiss (Jena, Germany) has introduced ELYRA P.1, a new module that makes possible three-dimensional (3D) super-resolution photoactivated localization microscopy (PALM) for endogenously-expressed photo-switchable fluorescent proteins.

In PALM, photo-switchable fluorescent molecules are sparsely activated so that only one out of many will be in its on-state within a single point spread function (PSF). In 3D, the PSF shape codes for the z-position. The localizations are plotted in a new image to create the super-resolved image. ELYRA P.1 achieves resolutions in the range of 20–30 nanometers laterally and 50–80 nanometers axially. The ELYRA P.1 module captures highly resolved structures in 3D, while treating the sample so gently that it remains available for long-term observation.

The ELYRA system can be integrated with the Zeiss laser scanning microscopes LSM 710 or LSM 780. In addition, ELYRA works seamlessly together with Zeiss scanning electron microscopes.

“With ELYRA, researchers can investigate the structural arrangement of one or multiple proteins, reveal the ultrastructure of cell organelles in 2D and 3D as well as map and count molecules within a structure. Sophisticated algorithms relate photon statistics to precision information in all directions, so researchers can display their structures fully rendered in 3D,” said Dr. Klaus Weisshart, ELYRA product manager at Zeiss.

Related Links:

Zeiss



comments powered by Disqus

Channels

Genomics/Proteomics

view channel
Image: Blocking the activity of HSP101 may imprison the malaria parasite inside its protective vacuole within the red blood cell. In the electron micrograph, the malaria parasites appear in blue and uninfected red blood cells are shown in red (Photo courtesy of the [US] National Institute of Allergy and Infectious Diseases).

Heat Shock Protein Plays Critical Role in Malaria Parasite Protein Trafficking

A pair of recent papers described the molecular operators that enable the malaria parasite Plasmodium falciparum to export a large variety of proteins across the parasitophorous vacuolar membrane (PVM)... Read more

Drug Discovery

view channel
Image: Molecular rendering of the crystal structure of parkin (Photo courtesy of Wikimedia Commons).

Cinnamon Feeding Blocks Development of Parkinson's Disease in Mouse Model

A team of neurological researchers has identified a molecular mechanism by which cinnamon acts to protect neurons from damage caused by Parkinson's disease (PD) in a mouse model of the syndrome.... Read more

Therapeutics

view channel
Image: This type of electronic pacemaker could become obsolete if induction of biological pacemaker cells by gene therapy proves successful (Photo courtesy of Wikimedia Commons).

Gene Therapy Induces Functional Pacemaker Cells in Pig Heart Failure Model

Cardiovascular disease researchers working with a porcine heart failure model have demonstrated the practicality of using gene therapy to replace implanted electronic pacemakers to regulate heartbeat.... Read more

Business

view channel

Cancer Immunotherapy Sector Predicted to Surge to USD 9 Billion Across Major Pharma Through 2022

The immunotherapy market will experience substantial growth through 2022, increasing from USD 1.1 billion in 2012 to nearly USD 9 billion in 2022 (corresponding to 23.8% annual growth) in the United Kingdom, United States, France, Germany, Italy, Spain, and Japan, according to recent market research. This notable growth... Read more
 
Copyright © 2000-2014 Globetech Media. All rights reserved.