Features | Partner Sites | Information | LinkXpress
Sign In
GLOBETECH MEDIA
GLOBETECH PUBLISHING LLC
GLOBETECH PUBLISHING LLC

Novel Pig Model Closely Mimics Human Helicobacter pylori Infection

By BiotechDaily International staff writers
Posted on 08 Oct 2013
Image: Similar anatomical properties between the stomach of humans and pigs may facilitate study of H. pylori-associated disease. Virginia Tech researchers have demonstrated that H. pylori (arrow) is found in the inner lining of the stomach and near aggregates of immune cells. The picture insert shows the typically spindle-shaped H. pylori magnified 1,000 times (Photo courtesy of Virginia Tech).
Image: Similar anatomical properties between the stomach of humans and pigs may facilitate study of H. pylori-associated disease. Virginia Tech researchers have demonstrated that H. pylori (arrow) is found in the inner lining of the stomach and near aggregates of immune cells. The picture insert shows the typically spindle-shaped H. pylori magnified 1,000 times (Photo courtesy of Virginia Tech).
Researchers have developed a novel pig model of Helicobacter pylori infection that closely mimics human gastric pathology and presents an avenue for studying human effector and regulatory responses toward H. pylori.

H. pylori are helix-shaped, Gram-negative bacteria, about three micrometers long with a diameter of about 0.5 micrometers. They are microaerophilic, requiring oxygen but at a lower concentration than is found in the atmosphere. H. pylori contain a hydrogenase, which can be used to obtain energy by oxidizing molecular hydrogen produced by intestinal bacteria. While more than 50% of the world's population harbor H. pylori in their upper gastrointestinal tract, over 80% of people infected with H. pylori show no symptoms. Acute infection may appear as an acute gastritis with abdominal pain or nausea. Where this develops into chronic gastritis, the symptoms, if present, are often those of non-ulcer dyspepsia: stomach pains, nausea, bloating, belching, and sometimes vomiting or black stool. Individuals infected with H. pylori have a 10% to 20% lifetime risk of developing peptic ulcers and a 1% to 2% risk of acquiring stomach cancer.

Small animals such as mice and gerbils have been used as models for H. pylori infection. However, the immune response generated by these animals was found to differ significantly from that of humans. In a paper published in the October 2013 issue of the journal Infection and Immunity investigators at Virginia Tech (Blacksburg, VA, USA) described a novel pig model for studying H. pylori infection.

Similar to the situation in humans, when pigs were infected with H. pylori, the animals generated an increase in pro-inflammatory CD4+ T helper cells, followed by an increase in CD8+ cytotoxic T cells. Marked rises were found in the levels of mRNA needed by peripheral blood mononuclear cells (PBMCs) for the production of inflammatory proteins such as gamma interferon, perforin, and granzyme.

“Pigs have greater anatomic, physiologic, and immunologic similarities to humans than mice, the main animal model used in biomedical research,” said senior author Dr. Raquel Hontecillas, assistant professor of immunology at Virginia Tech. “The results from our new pig model closely mimic what has been reported in clinical settings, which will allow us to comprehensively and systematically investigate human immune responses to H. pylori.”

Related Links:
Virginia Tech


Channels

Genomics/Proteomics

view channel
Image: Transmission electron micrograph of norovirus particles in feces (Photo courtesy of Wikimedia Commons).

Norovirus Interacts with Gut Bacteria to Establish a Persistent Infection That Can Be Blocked by Interferon Lambda

A team of molecular microbiologists and virologists has found that norovirus requires an intimate interaction with certain gut bacteria to establish a persistent infection, and that the infective process... Read more

Biochemistry

view channel

Possible New Target Found for Treating Brain Inflammation

Scientists have identified an enzyme that produces a class of inflammatory lipid molecules in the brain. Abnormally high levels of these molecules appear to cause a rare inherited eurodegenerative disorder, and that disorder now may be treatable if researchers can develop suitable drug candidates that suppress this enzyme.... Read more

Therapeutics

view channel
Image: Cancer cells infected with tumor-targeted oncolytic virus (red). Green indicates alpha-tubulin, a cell skeleton protein. Blue is DNA in the cancer cell nuclei (Photo courtesy of Dr. Rathi Gangeswaran, Bart’s Cancer Institute).

Innovative “Viro-Immunotherapy” Designed to Kill Breast Cancer Cells

A leading scientist has devised a new treatment that employs viruses to kill breast cancer cells. The research could lead to a promising “viro-immunotherapy” for patients with triple-negative breast cancer,... Read more

Business

view channel

Program Designed to Provide High-Performance Computing Cluster Systems for Bioinformatics Research

Dedicated Computing (Waukesha, WI, USA), a global technology company, reported that it will be participating in the Intel Cluster Ready program to deliver integrated high-performance computing cluster solutions to the life sciences market. Powered by Intel Xeon processors, Dedicated Computing is providing a range of... Read more
 
Copyright © 2000-2015 Globetech Media. All rights reserved.