Features | Partner Sites | Information | LinkXpress
Sign In
PZ HTL SA
GLOBETECH PUBLISHING
GLOBETECH PUBLISHING

Different Bacteria Cultures Maintained in Microfluidic System

By BiotechDaily International staff writers
Posted on 13 Aug 2013
Image: Microfluidic system for multiple bacterial cultures (Photo courtesy of Polish Institute of Physical Chemistry).
Image: Microfluidic system for multiple bacterial cultures (Photo courtesy of Polish Institute of Physical Chemistry).
A fabricated microfluidic device allows hundreds of different bacteria cultures to be maintained simultaneously.

The system allows for the merging, transporting and splitting of microdroplets where strictly controlled chemical reactions and the cultivation of bacterial colonies can be performed.

A group of scientists at the Institute of Physical Chemistry (Warsaw, Poland) engineered the microfluidic systems from polymer plates that correspond to the size of a credit card. Inside the system, a carrier fluid, mostly oil, carries microdroplets containing chemicals, flows laminarly through tiny channels of diameters in the range of tenths or hundredths of a millimeter. In this single microfluidic system thousands of different chemical reactions can be carried out during a day.

The microsystem is composed of two branches of microchannels forming densely arranged zigzags. A few hundred droplets can circulate in the microchannels, at a distance of about one centimeter from each other. The microdroplets move in a pendular movement from one branch to the other. Each droplet circulating within the microfluidic system has its own unique identifier, assigned by the optoelectronic system. It allows the scientist to monitor at any time what operations have been carried out on each microdroplet. A single droplet can include over 100,000 bacteria that are unable to move between the droplets, as the bacteria cannot cross the surface membrane of a microdroplet, and the carrier liquid used to transport microdroplets is not an environment favorable for the life of bacteria.

Piotr Garstecki, PhD, DSc, the lead author, said, “We can transform each microdroplet into a real bioreactor. Therefore, in a single small plate we can have up to several hundreds of bioreactors, with different, controlled concentration of an antibiotic, a different antibiotic or even different bacterial species in each bioreactor.” The paper was published on July 15, 2013, in the journal Angewandte Chemie International Edition.

Related Links:

Polish Institute of Physical Chemistry



Channels

Genomics/Proteomics

view channel
Image: The TheraCyte cell encapsulation device (Photo courtesy of TheraCyte, Inc.).

Encapsulated Human-Insulin-Producing Progenitor Cells Cure Diabetes in Mouse Model

A breakthrough system that allows subcutaneous implantation of encapsulated immature pancreatic cells (beta progenitor cells) was shown to produce enough insulin to correct the symptoms of diabetes in a mouse model.... Read more

Drug Discovery

view channel
Image: Chitosan is derived from the shells of shrimp and other sea crustaceans, including Alaskan pink shrimp, pictured here (Photo courtesy of NOAA - [US] National Oceanic and Atmospheric Administration).

Chitosan Treatment Clears the Way for Antibiotics to Eliminate Recurrent Urinary Tract Infections

Recurrent urinary tract infection was successfully resolved in a mouse model by treatment with the exfoliant chitosan followed by a round of antibiotics. Bacterial urinary tract infection (UTI), most... Read more

Biochemistry

view channel

Mitochondrial Cause of Aging Can Be Reversed

Researchers have found a cause of aging in lab animals that can be reversed, possibly providing an avenue for new treatments for age-related diseases including type 2 diabetes, cancer, muscle wasting, and inflammatory diseases. The researchers plan to begin human trials late 2014. The study, which was published December... Read more

Therapeutics

view channel

Cytokine Identified That Causes Mucositis in Cancer Therapy Patients

The action of the cytokine interleukin 1-beta (IL-1beta) has been found to underlie the onset of mucositis, a common, severe side effect of chemotherapy and irradiation of cancer patients. Mucositis occurs as a result of cell death in reaction to chemo- or radiotherapy. The mucosal lining of the mouth becomes thin, may... Read more

Business

view channel

Analytical Sciences Trade Fair Declared a Rousing Success

Organizers of this year's 24th "analytica" biosciences trade fair have reported significant increases in both the number of visitors and exhibitors compared to the 2012 event. The analytica trade fair for laboratory technology, analysis, and biotechnology has been held at the Munich (Germany) Trade Fair Center every... Read more
 
Copyright © 2000-2014 Globetech Media. All rights reserved.