Features | Partner Sites | Information | LinkXpress
Sign In
GLOBETECH PUBLISHING LLC
GLOBETECH MEDIA
GLOBETECH PUBLISHING LLC

Different Bacteria Cultures Maintained in Microfluidic System

By BiotechDaily International staff writers
Posted on 13 Aug 2013
Image: Microfluidic system for multiple bacterial cultures (Photo courtesy of Polish Institute of Physical Chemistry).
Image: Microfluidic system for multiple bacterial cultures (Photo courtesy of Polish Institute of Physical Chemistry).
A fabricated microfluidic device allows hundreds of different bacteria cultures to be maintained simultaneously.

The system allows for the merging, transporting and splitting of microdroplets where strictly controlled chemical reactions and the cultivation of bacterial colonies can be performed.

A group of scientists at the Institute of Physical Chemistry (Warsaw, Poland) engineered the microfluidic systems from polymer plates that correspond to the size of a credit card. Inside the system, a carrier fluid, mostly oil, carries microdroplets containing chemicals, flows laminarly through tiny channels of diameters in the range of tenths or hundredths of a millimeter. In this single microfluidic system thousands of different chemical reactions can be carried out during a day.

The microsystem is composed of two branches of microchannels forming densely arranged zigzags. A few hundred droplets can circulate in the microchannels, at a distance of about one centimeter from each other. The microdroplets move in a pendular movement from one branch to the other. Each droplet circulating within the microfluidic system has its own unique identifier, assigned by the optoelectronic system. It allows the scientist to monitor at any time what operations have been carried out on each microdroplet. A single droplet can include over 100,000 bacteria that are unable to move between the droplets, as the bacteria cannot cross the surface membrane of a microdroplet, and the carrier liquid used to transport microdroplets is not an environment favorable for the life of bacteria.

Piotr Garstecki, PhD, DSc, the lead author, said, “We can transform each microdroplet into a real bioreactor. Therefore, in a single small plate we can have up to several hundreds of bioreactors, with different, controlled concentration of an antibiotic, a different antibiotic or even different bacterial species in each bioreactor.” The paper was published on July 15, 2013, in the journal Angewandte Chemie International Edition.

Related Links:

Polish Institute of Physical Chemistry



Channels

Genomics/Proteomics

view channel
Image: Typical antibodies (left) unfold in the harsh environment of the cell. Camelid antibodies (right) are smaller and more stable (Photo courtesy of the Washington University School of Medicine).

Double Targeting Approach Increases Potential for Cancer Treatment with Oncolytic Viruses

Cancer researchers have used a double targeting approach to direct oncolytic viruses specifically to tumor cells where they reproduce until the cancer cells burst, releasing more viruses to infect and... Read more

Drug Discovery

view channel
Image: Molecular model of the protein Saposin C (Photo courtesy of Wikimedia Commons).

Nanovesicles Kill Human Lung Cancer Cells in Culture and in a Mouse Xenograft Model

Nanovesicles assembled from the protein Saposin C (SapC) and the phospholipid dioleoylphosphatidylserine (DOPS) were shown to be potent inhibitors of lung cancer cells in culture and in a mouse xenograft model.... Read more

Biochemistry

view channel

Possible New Target Found for Treating Brain Inflammation

Scientists have identified an enzyme that produces a class of inflammatory lipid molecules in the brain. Abnormally high levels of these molecules appear to cause a rare inherited eurodegenerative disorder, and that disorder now may be treatable if researchers can develop suitable drug candidates that suppress this enzyme.... Read more

Lab Technologies

view channel
Image: The FLUOVIEW FVMPE-RS Gantry microscope (Photo courtesy of Olympus).

New Multiphoton Laser Scanning Microscope Configurations Expand Research Potential

Two new configurations of a state-of-the-art multiphoton laser scanning microscope extend the usefulness of the instrument for examining rapidly occurring biological events and for obtaining images from... Read more

Business

view channel

Roche Acquires Signature Diagnostics to Advance Translational Research

Roche (Basel, Switzerland) will advance translational research for next generation sequencing (NGS) diagnostics by leveraging the unique expertise of Signature Diagnostics AG (Potsdam, Germany) in biobanks and development of novel NGS diagnostic assays. Signature Diagnostics is a privately held translational oncology... Read more
 
Copyright © 2000-2015 Globetech Media. All rights reserved.