Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
RANDOX LABORATORIES

Events

10 Oct 2016 - 12 Oct 2016
12 Nov 2016 - 16 Nov 2016

New Kit with Novel Method Improves Microbiome DNA Enrichment

By BiotechDaily International staff writers
Posted on 22 May 2013
Print article
A new kit has been developed to enrich microbial genomic DNA to enable more feasible and affordable microbiome next generation DNA sequencing.

New England Biolabs (NEB; Ipswich, MA, USA) introduces the “NEBNext Microbiome DNA Enrichment Kit,” which uses a method designed to separate microbial DNA from host DNA, reducing the high cost of sequencing microbiome DNA to a more practical level. Microbiome samples are commonly dominated by host DNA (up to 99%), complicating genetic analyses of these samples, particularly total microbiome DNA sequencing studies. Since only a small percentage of sequencing reads pertain to the microbes of interest, obtaining sufficient sequence coverage of the microbiome DNA can become cost-prohibitive or even technically infeasible.

The new kit utilizes the MBD2-Fc protein, which binds to CpG-methylated DNA (including human genomic DNA) with very high specificity. The MDB2-Fc protein is attached to Protein A-coated magnetic beads, enabling simple and quick removal of the contaminating host DNA in about 30 minutes. The microbial DNA-enriched sample is then ready to be processed for multiple downstream applications, including next generation sequencing, qPCR, and endpoint PCR.

“Although microbiome whole genome sequencing provides more information than other methods, such as 16S analysis, this method has proven difficult for many samples, due to the presence of contaminating host genomic DNA,” said Fiona Stewart, Product Marketing Manager, Next Generation Sequencing at NEB. “The NEBNext Microbiome DNA Enrichment Kit makes it possible to substantially enrich samples for nonhost, microbial DNA, while retaining microbial diversity, thereby improving the quality and cost-effectiveness of downstream analyses.”

Related Links:

New England Biolabs
NEBNext Microbiome DNA Enrichment Kit




Print article

Channels

Drug Discovery

view channel
Image: Ginger is the source of a novel class of nanolipid transport vector (Photo courtesy of Georgia State University).

Ginger-Derived Doxorubicin-Loaded Nanovectors as Drug Delivery for Cancer Therapy

A novel type of nanoparticle drug transport system based on lipids isolated from ginger was used to deliver the toxic chemotherapeutic agent doxorubicin (Dox) to colon cancer cells with minimal damage... Read more

Biochemistry

view channel
Image: A space-filling model of the anticonvulsant drug carbamazepine (Photo courtesy of Wikimedia Commons).

Wastewater May Contaminate Crops with Potentially Dangerous Pharmaceuticals

Reclaimed wastewater used to irrigate crops is contaminated with pharmaceutical residues that can be detected in the urine of those who consumed such produce. Investigators at the Hebrew University... Read more

Business

view channel

Collaborative Agreement to Aid in Setting Guidelines for Evaluating Potential Ebola Therapy

Cooperation between an Israeli biopharmaceutical company and medical branches of the US government is designed to set ground rules for continued evaluation of an experimental therapy for Ebola virus disease. RedHill Biopharma Ltd. (Tel Aviv, Israel), a biopharmaceutical company primarily focused on development and c... Read more
Copyright © 2000-2016 Globetech Media. All rights reserved.