Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
RANDOX LABORATORIES

New Kit with Novel Method Improves Microbiome DNA Enrichment

By BiotechDaily International staff writers
Posted on 22 May 2013
Print article
A new kit has been developed to enrich microbial genomic DNA to enable more feasible and affordable microbiome next generation DNA sequencing.

New England Biolabs (NEB; Ipswich, MA, USA) introduces the “NEBNext Microbiome DNA Enrichment Kit,” which uses a method designed to separate microbial DNA from host DNA, reducing the high cost of sequencing microbiome DNA to a more practical level. Microbiome samples are commonly dominated by host DNA (up to 99%), complicating genetic analyses of these samples, particularly total microbiome DNA sequencing studies. Since only a small percentage of sequencing reads pertain to the microbes of interest, obtaining sufficient sequence coverage of the microbiome DNA can become cost-prohibitive or even technically infeasible.

The new kit utilizes the MBD2-Fc protein, which binds to CpG-methylated DNA (including human genomic DNA) with very high specificity. The MDB2-Fc protein is attached to Protein A-coated magnetic beads, enabling simple and quick removal of the contaminating host DNA in about 30 minutes. The microbial DNA-enriched sample is then ready to be processed for multiple downstream applications, including next generation sequencing, qPCR, and endpoint PCR.

“Although microbiome whole genome sequencing provides more information than other methods, such as 16S analysis, this method has proven difficult for many samples, due to the presence of contaminating host genomic DNA,” said Fiona Stewart, Product Marketing Manager, Next Generation Sequencing at NEB. “The NEBNext Microbiome DNA Enrichment Kit makes it possible to substantially enrich samples for nonhost, microbial DNA, while retaining microbial diversity, thereby improving the quality and cost-effectiveness of downstream analyses.”

Related Links:

New England Biolabs
NEBNext Microbiome DNA Enrichment Kit




Print article

Channels

Genomics/Proteomics

view channel
Image: The green-labeled cells show a basal cell carcinoma in mouse tail epidermis derived from a single mutant stem cell and expanding out of the normal epidermis stained in red (Photo courtesy of Adriana Sánchez-Danés, Université Libre de Bruxelles).

Stem Cells Not Progenitors Can Trigger Skin Cancer Growth

Cancer researchers have discovered that stem cells can initiate development of malignant skin tumors, while progenitor cells are limited to triggering only benign growths. A progenitor cell is similar... Read more

Biochemistry

view channel
Image: A space-filling model of the anticonvulsant drug carbamazepine (Photo courtesy of Wikimedia Commons).

Wastewater May Contaminate Crops with Potentially Dangerous Pharmaceuticals

Reclaimed wastewater used to irrigate crops is contaminated with pharmaceutical residues that can be detected in the urine of those who consumed such produce. Investigators at the Hebrew University... Read more

Business

view channel

Sartorius Acquires US Start-up ViroCyt

Sartorius AG (Göttingen, Germany), a pharmaceutical and laboratory equipment provider, has acquired ViroCyt Incorporated (Broomfield, CO, USA), a start-up in the field of rapid virus quantification, in a deal valued at approximately USD 16 million. ViroCyt’s automated platform integrates instruments, software and reagents... Read more
Copyright © 2000-2016 Globetech Media. All rights reserved.