Features | Partner Sites | Information | LinkXpress
Sign In
BioConferenceLive
GLOBETECH PUBLISHING
JIB

New Plasmids Developed for Real-Time Monitoring of Key Intracellular Molecules

By BiotechDaily International staff writers
Posted on 28 Mar 2013
New plasmids are now available for real-time assays enabling cell biology as well as drug discovery researchers to visualize and study the in vivo action of target molecules involved in key regulatory cell processes.

ChromoTek GmbH (Munich, Germany) offers the new nanobody based “Chromobody Plasmids” to visualize and measure the action of candidate drugs or other substances of interest on important regulatory processes in living cells. Presently, four plasmids are available: for analyzing the cell cycle, apoptosis, DNA methylation, and cytoskeleton dynamics. A major advantage of the new Chromobody plasmids compared to Chromobody cell lines already available is that researchers can now monitor cellular pathways in their own cells or cell lines simply by carrying out a transfection. Unlike conventional, end-point assays, the Chromobody plasmids allow the fate of endogeneous target proteins to be followed over time, also visually in a movie-like format. Additionally, as the fluorescently labeled intracellular antibodies expressed from these plasmids do not interfere with the activity of their targets, they are extremely useful for high-content screening.

The new product format is equally suitable for academic researchers who want to decipher cellular pathways and for biotech and pharma researchers seeking to enhance their drug discovery and high-throughput screening capabilities. “We are glad to be able to offer our customers a new product format that gives them greater flexibility both in basic cell biology research and in drug discovery and validation processes,” stated Marion Jung, Managing Director of ChromoTek. Researchers who would like to try the Chromobody technology for their own applications can now choose between three options: license a cell line; order assay-ready frozen instant cells via ChromoTek or via CCS (Cell Culture Service, now part of Evotec AG); or purchase a Chromobody plasmid.

Related Links:

ChromoTek



comments powered by Disqus

Channels

Genomics/Proteomics

view channel
Image: In the liver tissue of obese animals with type II diabetes, unhealthy, fat-filled cells are prolific (small white cells, panel A). After chronic treatment through FGF1 injections, the liver cells successfully lose fat and absorb sugar from the bloodstream (small purple cells, panel B) and more closely resemble cells of normal, non-diabetic animals (Photo courtesy of the Salk Institute for Biological Studies).

Fibroblast Growth Factor 1 Treatment Restores Glucose Control in Mouse Diabetes Model

A "vaccine" based on the metabolic regulator fibroblast growth factor 1 (FGF1) removed the insulin resistance that characterizes type II diabetes and restored the body's natural ability to manage its glucose... Read more

Drug Discovery

view channel
Image: Molecular rendering of the crystal structure of parkin (Photo courtesy of Wikimedia Commons).

Cinnamon Feeding Blocks Development of Parkinson's Disease in Mouse Model

A team of neurological researchers has identified a molecular mechanism by which cinnamon acts to protect neurons from damage caused by Parkinson's disease (PD) in a mouse model of the syndrome.... Read more

Therapeutics

view channel
Image: This type of electronic pacemaker could become obsolete if induction of biological pacemaker cells by gene therapy proves successful (Photo courtesy of Wikimedia Commons).

Gene Therapy Induces Functional Pacemaker Cells in Pig Heart Failure Model

Cardiovascular disease researchers working with a porcine heart failure model have demonstrated the practicality of using gene therapy to replace implanted electronic pacemakers to regulate heartbeat.... Read more

Business

view channel

Cancer Immunotherapy Sector Predicted to Surge to USD 9 Billion Across Major Pharma Through 2022

The immunotherapy market will experience substantial growth through 2022, increasing from USD 1.1 billion in 2012 to nearly USD 9 billion in 2022 (corresponding to 23.8% annual growth) in the United Kingdom, United States, France, Germany, Italy, Spain, and Japan, according to recent market research. This notable growth... Read more
 
Copyright © 2000-2014 Globetech Media. All rights reserved.