Features | Partner Sites | Information | LinkXpress
Sign In
GLOBETECH PUBLISHING
PZ HTL SA
GLOBETECH PUBLISHING

Enhanced Characterization Technology Provides Better Understanding of Shape, Structure of Proteins

By BiotechDaily International staff writers
Posted on 20 Feb 2013
Image: The Viscotek TDA at Hamburg EMBL is used to enhance protein characterization by small angle X-ray scattering (SAXS) (Photo courtesy of Malvern Instruments).
Image: The Viscotek TDA at Hamburg EMBL is used to enhance protein characterization by small angle X-ray scattering (SAXS) (Photo courtesy of Malvern Instruments).
A triple detector array system used before protein analysis provides complementary biophysical data and simultaneously purifies samples for measurement, resulting in more data and better quality information from each experiment.

Researchers from the European Molecular Biology Laboratory (EMBL; Hamburg, Germany), have integrated a multidetector array size exclusion chromatography (SEC) system from Malvern Instruments (Viscotek TDA triple detector array; Malvern, Worchester, UK) at the laboratory’s strong, small angle X-ray scattering (SAXS) beamline to improve the productivity and quality of detailed protein characterization work.

This novel set-up is not only limited to the institute itself, but is widely accessible to structural biology researchers via BioStructX, a European Commission initiative that creates a single gateway to Europe’s leading synchrotron facilities.

SEC generates a fractionated sample for measurement, separated on the basis of hydrodynamic size. The Viscotek TDA integrates refractive index, ultraviolet (UV), and light scattering detectors, which in combination provide concentration and absolute molecular weight measurement for these eluting fractions. Incorporating this system with the SAXS beamline provides purified samples for measurement that provide optimal signal-to-noise ratios and high quality data. In combination with the biophysical information from the TDA, these data provide detailed evidence about the internal shape and structure of proteins that furthers understanding of their behavior. Specifically, SAXS information is helpful for the determination and tracking of the low-resolution structures and conformational alterations that proteins exhibit.

Both EMBL Hamburg and Malvern Instruments are partners in the BioStructX project, which brings together facilities from across Europe to establish a cutting-edge coordinated and multisite infrastructure to support access for key methods in structural biology. The new integrated SEC-SAXS set-up was developed by a number of specialists within EMBL, and with input from Malvern Instruments to enhance the department’s systems to BioSAXS users transnationally. The automated multipronged characterization has already demonstrated its advantages to the first users and it is expected to be in demand as the BioSructX project advances.

Malvern provides materials and biophysical characterization technology and expertise that enable scientists and engineers to determine and control the properties of dispersed systems. These systems range from proteins and polymers in solution, particle and nanoparticle suspensions, and emulsions, through to sprays and aerosols, industrial bulk powders and high concentration slurries.

Related Links:
European Molecular Biology Laboratory
Malvern Instruments


Channels

Genomics/Proteomics

view channel
Image: Biopsy of small bowel showing celiac disease manifested by blunting of villi, crypt hyperplasia, and lymphocyte infiltration of crypts (Photo courtesy of Wikimedia Commons).

Reduced Elafin Levels Associated with Celiac Disease Bowel Inflammation

Levels of the enzyme elafin, an endogenous serine protease inhibitor, were lower in the small intestinal epithelium of patients with active celiac disease (CD) as compared to similar tissue from control patients.... Read more

Drug Discovery

view channel

Retinoic Acid Prevents Precancerous Breast Cells from Progressing to Full-Blown Cancer

Retinoic acid, a derivative of vitamin A, was found to prevent pre-cancerous breast cells from progressing to full-blown cancer but did not have any effect on breast tumor cells. Investigators at Thomas Jefferson University (Philadelphia, PA, USA) worked with a novel breast cancer model that had been developed by treating... Read more

Biochemistry

view channel

Mitochondrial Cause of Aging Can Be Reversed

Researchers have found a cause of aging in lab animals that can be reversed, possibly providing an avenue for new treatments for age-related diseases including type 2 diabetes, cancer, muscle wasting, and inflammatory diseases. The researchers plan to begin human trials late 2014. The study, which was published December... Read more

Therapeutics

view channel

Cytokine Identified That Causes Mucositis in Cancer Therapy Patients

The action of the cytokine interleukin 1-beta (IL-1beta) has been found to underlie the onset of mucositis, a common, severe side effect of chemotherapy and irradiation of cancer patients. Mucositis occurs as a result of cell death in reaction to chemo- or radiotherapy. The mucosal lining of the mouth becomes thin, may... Read more

Business

view channel

Analytical Sciences Trade Fair Declared a Rousing Success

Organizers of this year's 24th "analytica" biosciences trade fair have reported significant increases in both the number of visitors and exhibitors compared to the 2012 event. The analytica trade fair for laboratory technology, analysis, and biotechnology has been held at the Munich (Germany) Trade Fair Center every... Read more
 
Copyright © 2000-2014 Globetech Media. All rights reserved.