Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
RANDOX LABORATORIES

Events

10 May 2016 - 16 May 2016
11 May 2016 - 13 May 2016

Enhanced Characterization Technology Provides Better Understanding of Shape, Structure of Proteins

By BiotechDaily International staff writers
Posted on 20 Feb 2013
Print article
Image: The Viscotek TDA at Hamburg EMBL is used to enhance protein characterization by small angle X-ray scattering (SAXS) (Photo courtesy of Malvern Instruments).
Image: The Viscotek TDA at Hamburg EMBL is used to enhance protein characterization by small angle X-ray scattering (SAXS) (Photo courtesy of Malvern Instruments).
A triple detector array system used before protein analysis provides complementary biophysical data and simultaneously purifies samples for measurement, resulting in more data and better quality information from each experiment.

Researchers from the European Molecular Biology Laboratory (EMBL; Hamburg, Germany), have integrated a multidetector array size exclusion chromatography (SEC) system from Malvern Instruments (Viscotek TDA triple detector array; Malvern, Worchester, UK) at the laboratory’s strong, small angle X-ray scattering (SAXS) beamline to improve the productivity and quality of detailed protein characterization work.

This novel set-up is not only limited to the institute itself, but is widely accessible to structural biology researchers via BioStructX, a European Commission initiative that creates a single gateway to Europe’s leading synchrotron facilities.

SEC generates a fractionated sample for measurement, separated on the basis of hydrodynamic size. The Viscotek TDA integrates refractive index, ultraviolet (UV), and light scattering detectors, which in combination provide concentration and absolute molecular weight measurement for these eluting fractions. Incorporating this system with the SAXS beamline provides purified samples for measurement that provide optimal signal-to-noise ratios and high quality data. In combination with the biophysical information from the TDA, these data provide detailed evidence about the internal shape and structure of proteins that furthers understanding of their behavior. Specifically, SAXS information is helpful for the determination and tracking of the low-resolution structures and conformational alterations that proteins exhibit.

Both EMBL Hamburg and Malvern Instruments are partners in the BioStructX project, which brings together facilities from across Europe to establish a cutting-edge coordinated and multisite infrastructure to support access for key methods in structural biology. The new integrated SEC-SAXS set-up was developed by a number of specialists within EMBL, and with input from Malvern Instruments to enhance the department’s systems to BioSAXS users transnationally. The automated multipronged characterization has already demonstrated its advantages to the first users and it is expected to be in demand as the BioSructX project advances.

Malvern provides materials and biophysical characterization technology and expertise that enable scientists and engineers to determine and control the properties of dispersed systems. These systems range from proteins and polymers in solution, particle and nanoparticle suspensions, and emulsions, through to sprays and aerosols, industrial bulk powders and high concentration slurries.

Related Links:
European Molecular Biology Laboratory
Malvern Instruments


Print article

Channels

Genomics/Proteomics

view channel
Image: An expression of NOTCH 1 (green color) in ACC stem cells (Photo courtesy of Yale University).

Adenoid Cystic Carcinoma Stem Cells Depend on NOTCH1 and SOX10 Signaling

Cancer researchers have isolated a stem cell population from the cells making up an adenoid cystic carcinoma (ACC) tumor and showed that the signaling factors NOTCH1 and SOX10 were essential for the cancer... Read more

Drug Discovery

view channel

Experimental Small-Molecule Anticancer Drug Blocks RAS-binding Domains

The experimental small-molecule anticancer drug rigosertib was shown to block tumor growth by acting as an RAS-mimetic and interacting with the RAS binding domains of RAF kinases, resulting in their inability to bind to RAS, which inhibited the RAS-RAF-MEK pathway. Oncogenic activation of RAS genes due to point mutations... Read more

Biochemistry

view channel
Image: A space-filling model of the anticonvulsant drug carbamazepine (Photo courtesy of Wikimedia Commons).

Wastewater May Contaminate Crops with Potentially Dangerous Pharmaceuticals

Reclaimed wastewater used to irrigate crops is contaminated with pharmaceutical residues that can be detected in the urine of those who consumed such produce. Investigators at the Hebrew University... Read more

Business

view channel

European Biotech Agreement to Promote Antigen-Drug Conjugation Technology

Two European biotech companies have joined forces to exploit and commercialize an innovative, site-specific ADC (antigen-drug conjugate) conjugation technology. ProBioGen (Berlin, Germany), a company specializing in the development and manufacture of complex glycoproteins and Eucodis Bioscience (Vienna, Austria), a... Read more
Copyright © 2000-2016 Globetech Media. All rights reserved.