Features | Partner Sites | Information | LinkXpress
Sign In
GLOBETECH PUBLISHING
JIB
GLOBETECH PUBLISHING

Enhanced Characterization Technology Provides Better Understanding of Shape, Structure of Proteins

By BiotechDaily International staff writers
Posted on 20 Feb 2013
Image: The Viscotek TDA at Hamburg EMBL is used to enhance protein characterization by small angle X-ray scattering (SAXS) (Photo courtesy of Malvern Instruments).
Image: The Viscotek TDA at Hamburg EMBL is used to enhance protein characterization by small angle X-ray scattering (SAXS) (Photo courtesy of Malvern Instruments).
A triple detector array system used before protein analysis provides complementary biophysical data and simultaneously purifies samples for measurement, resulting in more data and better quality information from each experiment.

Researchers from the European Molecular Biology Laboratory (EMBL; Hamburg, Germany), have integrated a multidetector array size exclusion chromatography (SEC) system from Malvern Instruments (Viscotek TDA triple detector array; Malvern, Worchester, UK) at the laboratory’s strong, small angle X-ray scattering (SAXS) beamline to improve the productivity and quality of detailed protein characterization work.

This novel set-up is not only limited to the institute itself, but is widely accessible to structural biology researchers via BioStructX, a European Commission initiative that creates a single gateway to Europe’s leading synchrotron facilities.

SEC generates a fractionated sample for measurement, separated on the basis of hydrodynamic size. The Viscotek TDA integrates refractive index, ultraviolet (UV), and light scattering detectors, which in combination provide concentration and absolute molecular weight measurement for these eluting fractions. Incorporating this system with the SAXS beamline provides purified samples for measurement that provide optimal signal-to-noise ratios and high quality data. In combination with the biophysical information from the TDA, these data provide detailed evidence about the internal shape and structure of proteins that furthers understanding of their behavior. Specifically, SAXS information is helpful for the determination and tracking of the low-resolution structures and conformational alterations that proteins exhibit.

Both EMBL Hamburg and Malvern Instruments are partners in the BioStructX project, which brings together facilities from across Europe to establish a cutting-edge coordinated and multisite infrastructure to support access for key methods in structural biology. The new integrated SEC-SAXS set-up was developed by a number of specialists within EMBL, and with input from Malvern Instruments to enhance the department’s systems to BioSAXS users transnationally. The automated multipronged characterization has already demonstrated its advantages to the first users and it is expected to be in demand as the BioSructX project advances.

Malvern provides materials and biophysical characterization technology and expertise that enable scientists and engineers to determine and control the properties of dispersed systems. These systems range from proteins and polymers in solution, particle and nanoparticle suspensions, and emulsions, through to sprays and aerosols, industrial bulk powders and high concentration slurries.

Related Links:
European Molecular Biology Laboratory
Malvern Instruments


comments powered by Disqus

Channels

Genomics/Proteomics

view channel
Image: To adhere to catheters and start urinary tract infections, bacteria extend microscopic fibers with sticky proteins at their ends. Researchers have developed a vaccine that blocks the EbpA protein, visible as a white bulge above, and stops infections in mice (Photo courtesy of Dr. John Heuser, Washington University School of Medicine).

Blocking Binding of Bacteria to Fibrinogen Prevents Biofilm Formation and Catheter-Associated Bladder Infection in Mice

A team of molecular microbiologists has identified and targeted a critical step in biofilm formation and developed a vaccine that prevents catheter-associated urinary tract infections in mice.... Read more

Drug Discovery

view channel
Image: S-649266 has more robust antibacterial activity than established antibiotics against multidrug-resistant bacteria (Photo courtesy of Shionogi).

Novel Antibiotic Shows Potential for Broad Range of Infections

The emergence of bacterial resistance to known antibacterial agents is becoming a major challenge in treating the infection caused by multi drug resistant (MDR) bacteria. In order to treat bacterial... Read more

Business

view channel

Collaboration of Mayo Clinic and IBM Cognitive Computer Devised to Improve Clinical Trial Research

The Mayo Clinic (Rochester, MN, USA) and IBM (Armonk, NY, USA) recently announced plans to pilot Watson, the IBM cognitive computer, to match patients more rapidly with suitable clinical trials. A proof-of-concept phase is currently ongoing, with the intent to introduce it into clinical use in early 2015.... Read more
 
Copyright © 2000-2014 Globetech Media. All rights reserved.