Features | Partner Sites | Information | LinkXpress
Sign In
GLOBETECH PUBLISHING LLC
GLOBETECH PUBLISHING LLC
PZ HTL SA

Optoelectrokinetic Tech Underlies New Biochip Approach

By BiotechDaily International staff writers
Posted on 05 Feb 2013
Image: Researchers have demonstrated a new technology that combines a laser and electric fields to create tiny centrifuge-like whirlpools to separate particles and microbes by size, a potential lab-on-a-chip system for medicine and research. Here the technique is used to collect a bacterium called Shewanella oneidensis (Photo courtesy of Purdue University).
Image: Researchers have demonstrated a new technology that combines a laser and electric fields to create tiny centrifuge-like whirlpools to separate particles and microbes by size, a potential lab-on-a-chip system for medicine and research. Here the technique is used to collect a bacterium called Shewanella oneidensis (Photo courtesy of Purdue University).
Image: Here the rapid electrokinetic patterning technique is used to arrange bacteria into a specific pattern. The technique may be used as a tool for nanomanufacturing because it shows promise for the assembly of suspended particles, called colloids. The ability to construct objects with colloids makes it possible to create structures with particular mechanical and thermal characteristics to manufacture electronic devices and tiny mechanical parts (Photo courtesy of Purdue University).
Image: Here the rapid electrokinetic patterning technique is used to arrange bacteria into a specific pattern. The technique may be used as a tool for nanomanufacturing because it shows promise for the assembly of suspended particles, called colloids. The ability to construct objects with colloids makes it possible to create structures with particular mechanical and thermal characteristics to manufacture electronic devices and tiny mechanical parts (Photo courtesy of Purdue University).
A recently developed optoelectrokinetic manipulation technique was used to separate microorganisms according to size, which demonstrated the potential of this method for the development of "lab-on-a-chip" diagnostic technologies.

The technique, rapid electrokinetic patterning - or REP, works by focusing an infrared laser to heat a fluid in a microchannel containing particles or bacteria. An electric field is applied that combined with the laser's heating action causes the fluid to circulate in a "microfluidic vortex" that functions like a centrifuge to isolate particles based on size.

A detailed description of the method, which was used to collect three different types of microorganisms: the bacterium Shewanella oneidensis MR-1; the single-cell spherical fungus Saccharomyces cerevisiae; and the spherical bacterium Staphylococcus aureus, was published in the December 7, 2012, issue of the journal Lab on a Chip.

Senior author Dr. Steven T. Wereley, professor of mechanical engineering at Purdue University (West Lafayette, IN, USA), said, "By properly choosing the electrical frequency we can separate blood components, such as platelets. Say you want to collect Shewanella bacteria, so you use a certain electrical frequency and collect them. Then the next day you want to collect platelets from blood. That is going to be a different frequency. We foresee the ability to dynamically select what you will collect, which you could not do with conventional tools. The new results demonstrate that REP can be used to sort biological particles but also that the technique is a powerful tool for development of a high-performance on-chip bioassay system."

"It will not be on the market in a year," said Dr. Wereley. "We are still in the research end of this. We are sort of at the stage of looking for the killer app for this technology."

The REP method may also have applications in the field of nanomanufacturing due to its ability to assemble suspensions of particles known as colloids. This may be particularly relevant to the pharmaceutical industry, as a number of drugs are manufactured from solid particles suspended in liquid. Currently particles are collected and separated from the liquid by a process of filtration and centrifugation—methods not now available for production use at the nano level.

Related Links:

Purdue University



comments powered by Disqus

Channels

Genomics/Proteomics

view channel
Image: This novel, flexible film that can react to light is a promising step toward an artificial retina (Photo courtesy of the American Chemical Society).

Novel Nanofilm May Be Artificial Retina Precursor

Researchers have used advanced nanotechnology techniques to develop a light-sensitive film that has potential for future artificial retina applications. Investigators at the Hebrew University of Jerusalem... Read more

Drug Discovery

view channel
Image: Disruption and removal of malaria parasites by the experimental drug (+)-SJ733 (Photo courtesy of the University of California, San Francisco).

Experimental Antimalaria Drug Induces the Immune System to Destroy Infected Red Blood Cells

An experimental drug for the treatment of malaria was found to induce morphological changes in infected erythrocytes that enabled the immune system to recognize and eliminate them. Investigators at... Read more

Biochemistry

view channel

Blocking Enzyme Switch Turns Off Tumor Growth in T-Cell Acute Lymphoblastic Leukemia

Researchers recently reported that blocking the action of an enzyme “switch” needed to activate tumor growth is emerging as a practical strategy for treating T-cell acute lymphoblastic leukemia. An estimated 25% of the 500 US adolescents and young adults diagnosed yearly with this aggressive disease fail to respond to... Read more

Business

view channel

R&D Partnership Initiated to Reduce Development Time for New Drugs

nanoPET Pharma, GmbH (Berlin, Germany) signed an open-ended framework contract with the international pharmaceutical company Boehringer Ingelheim (Ridgefield, CT, USA). By developing customized contrast agents for research in both basic and preclinical studies, nanoPET Pharma will contribute to the enhancement of Boehringer... Read more
 
Copyright © 2000-2014 Globetech Media. All rights reserved.