We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
PURITAN MEDICAL

Download Mobile App




New DNA-Based Assay Rapidly Detects E. coli O157:H7 During Food Manufacture

By LabMedica International staff writers
Posted on 18 Nov 2015
Print article
The ANSR (Amplified Nucleic Single Temperature Reaction) system
The ANSR (Amplified Nucleic Single Temperature Reaction) system (Photo courtesy of NEOGEN)
A rapid DNA-based test for the detection of the serious food borne pathogen E. coli O157:H7 joins a line of innovative assays designed for use by the food industry.

The ANSR E. coli O157:H7 kit is a welcome addition to Neogen Corporation’s (Lansing, MI, USA) ANSR tests for Salmonella, Listeria, and Listeria monocytogenes.

Neogen's ANSR (Amplified Nucleic Single Temperature Reaction) platform incorporates advances in DNA detection technology resulting in a method that is easy to use and provides much faster results than PCR systems. ANSR was designed to address in-plant testing needs and offers the freedom to economically process samples to meet regulatory requirements and ensure food safety.

ANSR uses isothermal amplification to create a level of molecular target material that can be detected using fluorescence. The system's DNA amplification methodology exponentially amplifies the DNA of any target bacteria present in an enriched food sample to detectable levels in only minutes. Target nucleic acid is amplified through a mechanism of polymerization from the ends of nicks created in double-stranded DNA by the action of a specific endonuclease. Amplified target sequences are detected in real time using fluorescent molecular beacon probes.

To date, the assay has been validated for testing raw ground beef, raw beef trim, leafy greens, and sprout irrigation water. The system has exhibited low matrix effects with high sensitivity and provides both positive and negative results in minutes.

"The well-received and validated ANSR system provides the food industry definitive test results, and much easier and quicker methodology," said Ed Bradley, vice president of food safety at Neogen. "This introduction of ANSR for E. coli O157:H7 extends our ANSR product offering for the bacterial pathogens of greatest concern to the food industry, and will be very easy for laboratories to adopt, as it follows the same procedure of all our other ANSR kits."

Related Links:

Neogen Corporation 


Platinum Member
COVID-19 Rapid Test
OSOM COVID-19 Antigen Rapid Test
HLX
Anti-Cyclic Citrullinated Peptide Test
GPP-100 Anti-CCP Kit
Gold Member
Xylazine Immunoassay Test
Xylazine ELISA
Read the full article by registering today, it's FREE! It's Free!
Register now for FREE to LabMedica.com and get complete access to news and events that shape the world of Clinical Laboratory Medicine.
  • Free digital version edition of LabMedica International sent by email on regular basis
  • Free print version of LabMedica International magazine (available only outside USA and Canada).
  • Free and unlimited access to back issues of LabMedica International in digital format
  • Free LabMedica International Newsletter sent every week containing the latest news
  • Free breaking news sent via email
  • Free access to Events Calendar
  • Free access to LinkXpress new product services
  • REGISTRATION IS FREE AND EASY!
Click here to Register








Channels

Clinical Chemistry

view channel
Image: Reaching speeds up to 6,000 RPM, this centrifuge forms the basis for a new type of inexpensive, POC biomedical test (Photo courtesy of Duke University)

POC Biomedical Test Spins Water Droplet Using Sound Waves for Cancer Detection

Exosomes, tiny cellular bioparticles carrying a specific set of proteins, lipids, and genetic materials, play a crucial role in cell communication and hold promise for non-invasive diagnostics.... Read more

Molecular Diagnostics

view channel
Image: The study showed the blood-based cancer screening test detects 83% of people with colorectal cancer with specificity of 90% (Photo courtesy of Guardant Health)

Blood Test Shows 83% Accuracy for Detecting Colorectal Cancer

Colorectal cancer is the second biggest cause of cancer deaths among adults in the U.S., with forecasts suggesting 53,010 people might die from it in 2024. While fewer older adults are dying from this... Read more

Hematology

view channel
Image: The Gazelle Hb Variant Test (Photo courtesy of Hemex Health)

First Affordable and Rapid Test for Beta Thalassemia Demonstrates 99% Diagnostic Accuracy

Hemoglobin disorders rank as some of the most prevalent monogenic diseases globally. Among various hemoglobin disorders, beta thalassemia, a hereditary blood disorder, affects about 1.5% of the world's... Read more

Microbiology

view channel
Image: The new platform is designed to perform blood-based diagnoses of nontuberculosis mycobacteria (Photo courtesy of 123RF)

New Blood Test Cuts Diagnosis Time for Nontuberculous Mycobacteria Infections from Months to Hours

Breathing in nontuberculous mycobacteria (NTM) is a common experience for many people. These bacteria are present in water systems, soil, and dust all over the world and usually don't cause any problems.... Read more

Industry

view channel
Image: These new assays are being developed for use on the recently introduced DxI 9000 Immunoassay Analyzer (Photo courtesy of Beckman Coulter)

Beckman Coulter and Fujirebio Expand Partnership on Neurodegenerative Disease Diagnostics

Beckman Coulter Diagnostics (Brea, CA, USA) and Fujirebio Diagnostics (Tokyo, Japan) have expanded their partnership focused on the development, manufacturing and clinical adoption of neurodegenerative... Read more
Copyright © 2000-2024 Globetech Media. All rights reserved.