Features | Partner Sites | Information | LinkXpress
Sign In
GLOBETECH PUBLISHING LLC
GLOBETECH PUBLISHING LLC
GLOBETECH MEDIA

Deletion of the FOXO1 Gene Transforms Intestinal Cells into Insulin Producers

By BiotechDaily International staff writers
Posted on 13 Jul 2014
Image: Human gastrointestinal cells from patients were engineered to express insulin (fluorescent green) in the laboratory (Photo courtesy of Columbia University).
Image: Human gastrointestinal cells from patients were engineered to express insulin (fluorescent green) in the laboratory (Photo courtesy of Columbia University).
After having demonstrated that gut endocrine progenitor cells of mice could be differentiated into glucose-responsive, insulin-producing cells by elimination of the transcription factor FOXO1 (forkhead box O1), diabetes researchers have extended these findings by obtaining similar results with human gut endocrine progenitor and serotonin-producing cells.

Generation of alternative sources of insulin-producing beta-cells has been a goal of researchers in the field of diabetes therapy. While most efforts have been directed at differentiating embryonic or induced pluripotent stem (iPS) cells into beta-like-cells, investigators at Columbia University (New York, NY, USA) have shown that shown that mouse intestinal cells could be transformed into insulin-producing cells by deactivating the cells’ FOXO1 gene.

In a paper published in the June 30, 2014, online edition of the journal Nature Communications, the investigators revealed that FOXO1 was present in human gut endocrine progenitor and serotonin-producing cells. Using gut organoids derived from human iPS cells, they demonstrated that inhibition of FOXO1 using a dominant-negative mutant or Lentivirus-encoded small hairpin RNA promoted generation of insulin-positive cells that expressed all markers of mature pancreatic beta-cells and survived in vivo following transplantation into mice.

“People have been talking about turning one cell into another for a long time, but until now we had not gotten to the point of creating a fully functional insulin-producing cell by the manipulation of a single target,” said senior author Dr. Domenico Accili, professor of diabetes at Columbia University. “By showing that human cells can respond in the same way as mouse cells, we have cleared a main hurdle and can now move forward to try to make this treatment a reality.”

Related Links:

Columbia University



Channels

Drug Discovery

view channel

Curcumin Used to Treat Alzheimer’s Disease

Curcumin, a natural substance found in the spice turmeric, has been used by many Asian cultures for centuries. Now, new research suggests that a close chemical analog of curcumin has properties that may make it useful as a treatment for Alzheimer’s disease. “Curcumin has demonstrated ability to enter the brain, bind... Read more

Biochemistry

view channel

Blocking Enzyme Switch Turns Off Tumor Growth in T-Cell Acute Lymphoblastic Leukemia

Researchers recently reported that blocking the action of an enzyme “switch” needed to activate tumor growth is emerging as a practical strategy for treating T-cell acute lymphoblastic leukemia. An estimated 25% of the 500 US adolescents and young adults diagnosed yearly with this aggressive disease fail to respond to... Read more

Therapeutics

view channel
Image: Cancer cells infected with tumor-targeted oncolytic virus (red). Green indicates alpha-tubulin, a cell skeleton protein. Blue is DNA in the cancer cell nuclei (Photo courtesy of Dr. Rathi Gangeswaran, Bart’s Cancer Institute).

Innovative “Viro-Immunotherapy” Designed to Kill Breast Cancer Cells

A leading scientist has devised a new treatment that employs viruses to kill breast cancer cells. The research could lead to a promising “viro-immunotherapy” for patients with triple-negative breast cancer,... Read more

Lab Technologies

view channel
Image: MIT researchers have designed a microfluidic device that allows them to precisely trap pairs of cells (one red, one green) and observe how they interact over time (Photo courtesy of Burak Dura, MIT).

New Device Designed to See Communication between Immune Cells

The immune system is a complicated network of many different cells working together to defend against invaders. Effectively combating an infection depends on the interactions between these cells.... Read more

Business

view channel

Program Designed to Provide High-Performance Computing Cluster Systems for Bioinformatics Research

Dedicated Computing (Waukesha, WI, USA), a global technology company, reported that it will be participating in the Intel Cluster Ready program to deliver integrated high-performance computing cluster solutions to the life sciences market. Powered by Intel Xeon processors, Dedicated Computing is providing a range of... Read more
 
Copyright © 2000-2015 Globetech Media. All rights reserved.