Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
RANDOX LABORATORIES

Fruit Fly Study Leads to New Understanding of How mRNA Regulates Gene Expression

By BiotechDaily International staff writers
Posted on 02 Apr 2014
Print article
A study on heat sensitivity in fruit flies has led to a new understanding of how gene expression is regulated by mRNAs in a fashion that is independent of both DNA and protein.

Investigators at the Washington University School of Medicine (St. Louis, MO, USA) were studying the genetic mechanism that controls the behavior of a mutant variety of Drosophila that was unusually sensitive to high temperatures. These flies carried a mutated copy of a gene called seizure (sei) that rendered them so sensitive to heat that a rise in temperature of even 10 degrees was sufficient to send them into seizures.

"When we looked at the sei gene, we noticed that there is another gene on the opposite strand of the double-stranded DNA molecule called pickpocket 29 (ppk29)," said senior author Dr. Yehuda Ben-Shahar, assistant professor of biology at the Washington University School of Medicine. "This was interesting because sei codes for a protein ‘gate’ that lets potassium ions out of the neuron and pickpocket 29 codes for a gate that lets sodium ions into the neuron."

The investigators generated a series of transgenic lines of Drosophila with different ratios of sei and ppk29. They reported in the March 18, 2014, online edition of the journal eLife that mRNA originating from ppk29 was regulating the mRNA from the sei gene. The regulatory component of ppk29 was identified as the untranslated 3' UTR end of the mRNA strand. When this section of ppk29 mRNA formed a double stranded segment with complementary DNA from sei it resulted in the destruction of the sei strand through the combination of the Dicer enzyme and RISC (RNA-induced silencing complex) assembly. Thus, the gene coding a sodium channel was found to regulate the expression of the potassium channel gene.

“Our findings show that mRNAS, which are typically thought to act solely as the template for protein translation, can also serve as regulatory RNAs, independent of their protein-coding capacity,” said Dr. Ben-Shahar. “They are not just messengers but also actors in their own right.”

Related Links:

Washington University School of Medicine



Print article

Channels

Biochemistry

view channel
Image: A space-filling model of the anticonvulsant drug carbamazepine (Photo courtesy of Wikimedia Commons).

Wastewater May Contaminate Crops with Potentially Dangerous Pharmaceuticals

Reclaimed wastewater used to irrigate crops is contaminated with pharmaceutical residues that can be detected in the urine of those who consumed such produce. Investigators at the Hebrew University... Read more

Lab Technologies

view channel
Image: A 3D nanofiber net formed by the supergelators to trap oil molecules (Photo courtesy of IBN at A*STAR / Institute of Bioengineering and Nanotechnology).

Effective Cleanup with Smart Material That Forms Oil-Trapping Net

Researchers have developed supergelators – an organic oil-scavenging material that rapidly forms a 3D net to trap oil molecules, gelatinizing into solidified masses that can be more easily removed from... Read more

Business

view channel

Collaborative Agreement to Aid in Setting Guidelines for Evaluating Potential Ebola Therapy

Cooperation between an Israeli biopharmaceutical company and medical branches of the US government is designed to set ground rules for continued evaluation of an experimental therapy for Ebola virus disease. RedHill Biopharma Ltd. (Tel Aviv, Israel), a biopharmaceutical company primarily focused on development and c... Read more
Copyright © 2000-2016 Globetech Media. All rights reserved.