Features | Partner Sites | Information | LinkXpress
Sign In
GLOBETECH MEDIA
GLOBETECH PUBLISHING LLC
GLOBETECH PUBLISHING LLC

Highly Aligned Nanofibers Developed for Tissue Engineering Applications

By BiotechDaily International staff writers
Posted on 10 Mar 2014
Image: Highly aligned nanofibers created by fibroblasts form biological scaffolding that could prove an ideal foundation for engineered tissues. Stem cells placed on the scaffolding thrived, and it had the added advantage of provoking a very low immune response (Photo courtesy of Dr. Feng Zhao, Michigan Technological University).
Image: Highly aligned nanofibers created by fibroblasts form biological scaffolding that could prove an ideal foundation for engineered tissues. Stem cells placed on the scaffolding thrived, and it had the added advantage of provoking a very low immune response (Photo courtesy of Dr. Feng Zhao, Michigan Technological University).
A team of biomedical engineers has constructed a uniform highly aligned nanoscale fibrous scaffolding from extracellular matrix (ECM) naturally deposited by fibroblasts to be used for potential tissue engineering applications.

When placed in contact with ECM obtained from epithelial cells or fibroblasts, cells attach quickly, exhibit high plating and cloning efficiencies, proliferate rapidly, reach a high saturation density, exhibit lower requirements for serum and added growth factors, respond better to physiologically occurring hormones, express differentiated functions, have longer life span, undergo flattening and morphological changes, and have better plating consistency. Such cells adopt growth characteristics, morphological appearance, and biological responses that are not expressed when the same cells are maintained on artificial substrata (plastic, glass), even if coated with isolated constituents of ECM such as purified collagen or glycoproteins.

Investigators at Michigan Technological University (Houghton, USA) used synthetic nanogratings (130 nanometers in depth) to direct the growth of human dermal fibroblasts for up to eight weeks, resulting in a uniform 70 micrometer-thick fibroblast cell sheet with highly aligned cells and ECM nanofibers. A natural ECM scaffolding with uniformly aligned nanofibers about 78 nanometers in diameter was generated after removing the cellular components from the fibroblast sheet.

In a report published in the January 29, 2014, online edition of the journal Advanced Functional Materials the investigators demonstrated the excellent capacity of the ECM-scaffolding in directing and supporting alignment and proliferation of reseeded human mesenchymal stem cells (hMSCs) along the underlying fibers. Furthermore, the aligned ECM scaffolding induced a significantly lower immune response compared to its unaligned counterpart, as detected by proinflammatory cytokines secreted from macrophages.

“The cells did the work,” said senior author Dr. Feng Zhao, assistant professor of biomedical engineering at Michigan Technological University. “The material they made is quite uniform, and of course it is completely biological. We think this has great potential. I think we could use this to engineer softer tissues, like skin, blood vessels, and muscle.”

Related Links:

Michigan Technological University



Channels

Drug Discovery

view channel
Image: Molecular model of the protein Saposin C (Photo courtesy of Wikimedia Commons).

Nanovesicles Kill Human Lung Cancer Cells in Culture and in a Mouse Xenograft Model

Nanovesicles assembled from the protein Saposin C (SapC) and the phospholipid dioleoylphosphatidylserine (DOPS) were shown to be potent inhibitors of lung cancer cells in culture and in a mouse xenograft model.... Read more

Biochemistry

view channel

Possible New Target Found for Treating Brain Inflammation

Scientists have identified an enzyme that produces a class of inflammatory lipid molecules in the brain. Abnormally high levels of these molecules appear to cause a rare inherited eurodegenerative disorder, and that disorder now may be treatable if researchers can develop suitable drug candidates that suppress this enzyme.... Read more

Lab Technologies

view channel
Image: The FLUOVIEW FVMPE-RS Gantry microscope (Photo courtesy of Olympus).

New Multiphoton Laser Scanning Microscope Configurations Expand Research Potential

Two new configurations of a state-of-the-art multiphoton laser scanning microscope extend the usefulness of the instrument for examining rapidly occurring biological events and for obtaining images from... Read more

Business

view channel

Roche Acquires Signature Diagnostics to Advance Translational Research

Roche (Basel, Switzerland) will advance translational research for next generation sequencing (NGS) diagnostics by leveraging the unique expertise of Signature Diagnostics AG (Potsdam, Germany) in biobanks and development of novel NGS diagnostic assays. Signature Diagnostics is a privately held translational oncology... Read more
 
Copyright © 2000-2015 Globetech Media. All rights reserved.