Features | Partner Sites | Information | LinkXpress
Sign In
PZ HTL SA
GLOBETECH PUBLISHING LLC
GLOBETECH PUBLISHING LLC

Stem Cell-Based Cerebral Organoids Enable in Vitro Study of Human Brain Development and Microcephaly

By BiotechDaily International staff writers
Posted on 09 Sep 2013
Image: Comparison of the organoid (right) to the developing brain (left, section of a mouse brain) (Photo courtesy of the Institute of Molecular Biotechnology of the Austrian Academy of Sciences).
Image: Comparison of the organoid (right) to the developing brain (left, section of a mouse brain) (Photo courtesy of the Institute of Molecular Biotechnology of the Austrian Academy of Sciences).
Austrian researchers have used advanced stem cell technology to grow cultures of cerebral organoids, or "mini-brains," which enable the in vitro study of human neuronal disorders.

The complexity of the human brain has made it difficult to study many brain disorders in model organisms, highlighting the need for an in vitro model of human brain development. Investigators at the Institute of Molecular Biotechnology of the Austrian Academy of Sciences (Vienna) have developed a human pluripotent stem cell-derived three-dimensional organoid culture system, termed cerebral organoids, which develop various discrete, although interdependent, brain regions. These include a cerebral cortex containing progenitor populations that organize and produce mature cortical neuron subtypes.

The investigators reported in the August 28, 2013, online edition of the journal Nature that after 15–20 days of culture growth cerebral organoids formed that consisted of continuous tissue (neuroepithelia) surrounding a fluid-filled cavity that was reminiscent of a cerebral ventricle. After 20–30 days, defined brain regions, including a cerebral cortex, retina, meninges, as well as choroid plexus, developed. After two months, the mini-brains reached a maximum size, but they could survive indefinitely (currently up to 10 months) in a spinning bioreactor. Further growth, however, was not achieved, most likely due to the lack of a circulation system and hence a lack of nutrients and oxygen at the core of the mini-brains

Cerebral organoids were shown to recapitulate features of human cortical development, namely characteristic progenitor zone organization with abundant outer radial glial stem cells. The investigators used RNA interference and patient-specific induced pluripotent stem cells to model microcephaly, a disorder that has been difficult to recapitulate in mice. They demonstrated premature neuronal differentiation in patient organoids, a defect that could help to explain the disease phenotype.

Senior author Dr. Jürgen Knoblich, deputy scientific director of the Institute of Molecular Biotechnology of the Austrian Academy of Sciences, said, "We modified an established approach to generate so-called neuroectoderm, a cell layer from which the nervous system derives. Fragments of this tissue were then maintained in a 3D-culture and embedded in droplets of a specific gel that provided a scaffold for complex tissue growth. In order to enhance nutrient absorption, we later transferred the gel droplets to a spinning bioreactor. Within three to four weeks defined brain regions were formed."

Related Links:
Institute of Molecular Biotechnology of the Austrian Academy of Sciences



comments powered by Disqus

Channels

Drug Discovery

view channel
Image: The nano-cocoon drug delivery system is biocompatible, specifically targets cancer cells, can carry a large drug load, and releases the drugs very quickly once inside the cancer cell. Ligands on the surface of the \"cocoon\" trick cancer cells into consuming it. Enzymes (the “worms\" in this image) inside the cocoon are unleashed once inside the cell, destroying the cocoon and releasing anticancer drugs into the cell (Photo courtesy of Dr. Zhen Gu, North Carolina State University).

Novel Anticancer Drug Delivery System Utilizes DNA-Based Nanocapsules

A novel DNA-based drug delivery system minimizes damage to normal tissues by utilizing the acidic microenvironment inside cancer cells to trigger the directed release of the anticancer drug doxorubicin (DOX).... Read more

Lab Technologies

view channel

Experimental Physicists Find Clues into How Radiotherapy Kills Cancer Cells

A new discovery in experimental physics has implications for a better determination of the process in which radiotherapy destroys cancer cells. Dr. Jason Greenwood from Queen’s University Belfast (Ireland) Center for Plasma Physics collaborated with scientists from Italy and Spain on the work on electrons, and published... Read more

Business

view channel

Interest in Commercial Applications for Proteomics Continues to Grow

Increasing interest in the field of proteomics has led to a series of agreements between private proteomic companies and academic institutions as well as deals between pharmaceutical companies and novel proteomics innovator biotech companies. Proteomics is the study of the structure and function of proteins.... Read more
 
Copyright © 2000-2014 Globetech Media. All rights reserved.