Features | Partner Sites | Information | LinkXpress
Sign In
GLOBETECH PUBLISHING LLC
GLOBETECH PUBLISHING LLC
GLOBETECH MEDIA

Blood Collection Device Minimizes Cellular DNA Release

By BiotechDaily International staff writers
Posted on 27 Aug 2013
Image: Cell-free DNA BCT tube (Photo courtesy of Streck, Inc.).
Image: Cell-free DNA BCT tube (Photo courtesy of Streck, Inc.).
Cell-free DNA (cfDNA) circulating in blood is currently used for noninvasive diagnostic and prognostic tests and minimizing background DNA is vital for detection of low abundance cfDNA.

A newly introduced blood collection device could reduce background levels of genomic DNA (gDNA) in plasma compared to standard tubes, when subjected to conditions that may occur during sample storage and shipping.

Scientists working for a commercial manufacturer took blood samples from healthy donors into tri-potassium ethylenediaminetetraacetic acid (K3EDTA) tubes and Cell-Free DNA BCT (BCT), which is a new collection device that contains a novel chemical cocktail. Samples were shaken or left unshaken to simulate shipping. In a shipping study, samples were shipped or not shipped. To assess temperature variations, samples were incubated at 6 °C, 22 °C, and 37 °C. In all cases, plasma was harvested by centrifugation and total plasma DNA (pDNA) assayed by quantitative real-time polymerase chain reaction (qPCR). The QIAamp Circulating Nucleic Acid Kit (Qiagen; Santa Clarita, CA, USA) was used for the extraction of pDNA.

The BCT tubes are manufactured by Streck, Inc., (Omaha, NE, USA) while the K3EDTA tubes were the BD Vacutainer, (Becton Dickinson; Franklin Lakes, NJ, USA). Shaking of blood drawn into K3EDTA tubes showed a significant increase in pDNA over 24 hours whereas no change was seen in Cell-Free DNA BCT tubes. Blood incubated at 6 °C, 22 °C, and 37 °C showed significant increases in pDNA isolated from K3EDTA tubes, while pDNA levels from Cell-Free DNA BCT remained stable over 14 days.

The novel stabilizing chemical cocktail prevents the release of gDNA into plasma post phlebotomy up to 14 days, avoiding labor-intensive requirements. Using this blood collection device, ex vivo storage at room temperature becomes possible, allowing flexibility for offsite blood drawn to be sent to centralized laboratories for downstream analysis of the cfDNA without preliminary centrifugations or cryopreservation.

The authors concluded that Cell-Free DNA BCT tubes prevent increases in background gDNA levels caused by temperature fluctuations or agitation that can occur during blood sample storage, shipping and processing. This novel blood-collection tube provides a method for obtaining high quality stabilized samples for rare DNA target detection and determining accurate cfDNA concentrations. The study was published on July 12, 2013, in the Journal of Clinical Laboratory Analysis.

Related Links:

Qiagen
Streck, Inc.
Becton Dickinson



Channels

Genomics/Proteomics

view channel
Image: Typical antibodies (left) unfold in the harsh environment of the cell. Camelid antibodies (right) are smaller and more stable (Photo courtesy of the Washington University School of Medicine).

Double Targeting Approach Increases Potential for Cancer Treatment with Oncolytic Viruses

Cancer researchers have used a double targeting approach to direct oncolytic viruses specifically to tumor cells where they reproduce until the cancer cells burst, releasing more viruses to infect and... Read more

Drug Discovery

view channel
Image: Molecular model of the protein Saposin C (Photo courtesy of Wikimedia Commons).

Nanovesicles Kill Human Lung Cancer Cells in Culture and in a Mouse Xenograft Model

Nanovesicles assembled from the protein Saposin C (SapC) and the phospholipid dioleoylphosphatidylserine (DOPS) were shown to be potent inhibitors of lung cancer cells in culture and in a mouse xenograft model.... Read more

Biochemistry

view channel

Possible New Target Found for Treating Brain Inflammation

Scientists have identified an enzyme that produces a class of inflammatory lipid molecules in the brain. Abnormally high levels of these molecules appear to cause a rare inherited eurodegenerative disorder, and that disorder now may be treatable if researchers can develop suitable drug candidates that suppress this enzyme.... Read more

Lab Technologies

view channel
Image: The FLUOVIEW FVMPE-RS Gantry microscope (Photo courtesy of Olympus).

New Multiphoton Laser Scanning Microscope Configurations Expand Research Potential

Two new configurations of a state-of-the-art multiphoton laser scanning microscope extend the usefulness of the instrument for examining rapidly occurring biological events and for obtaining images from... Read more

Business

view channel

Roche Acquires Signature Diagnostics to Advance Translational Research

Roche (Basel, Switzerland) will advance translational research for next generation sequencing (NGS) diagnostics by leveraging the unique expertise of Signature Diagnostics AG (Potsdam, Germany) in biobanks and development of novel NGS diagnostic assays. Signature Diagnostics is a privately held translational oncology... Read more
 
Copyright © 2000-2015 Globetech Media. All rights reserved.