Features | Partner Sites | Information | LinkXpress
Sign In
GLOBETECH PUBLISHING LLC
GLOBETECH PUBLISHING LLC
GLOBETECH MEDIA

"Transparent Brain" Expected to Yield Breakthroughs in Understanding Neurological Disorders

By BiotechDaily International staff writers
Posted on 25 Apr 2013
Replacement of the brain's fat content with a clear, permeable gel allows optical, fluorescent, and electron microscope studies as well as immunohistochemical analyses to be carried out on intact tissues that have not been damaged or modified by sample preparation techniques.

Investigators at Stanford University (Palo Alto, CA, USA) developed a novel method for creating a "transparent" brain by replacing fat tissue with a clear, permeable gel. The technique was based on infusing a cocktail of reagents, including a plastic-like polymer and formaldehyde, into a mouse brain. When heated, the solution formed a transparent, porous gel that biochemically integrated with, and physically supported, the brain tissue while excluding the lipids, which were removed via an electrochemical process. The process was named CLARITY for Clear Lipid-exchanged Anatomically Rigid Imaging/Immunostaining-compatible Tissue Hydrogel.

A report in the April 10, 2013, online edition of the journal Nature revealed initial results obtained with a CLARITY-treated mouse brain. These results showed intact-tissue imaging of long-range projections, local circuit wiring, cellular relationships, subcellular structures, protein complexes, nucleic acids, and neurotransmitters. CLARITY also enabled intact-tissue in situ hybridization, immunohistochemistry with multiple rounds of staining and de-staining in nonsectioned tissue, and antibody labeling throughout the intact adult mouse brain.

In addition, CLARITY enabled fine structural analysis of clinical samples, including nonsectioned human tissue from a formaldehyde-preserved postmortem human brain from a person who had autism, establishing a path for the transmutation of human tissue into a stable, intact, and accessible form suitable for probing structural and molecular underpinnings of physiological function and disease.

“CLARITY will help support integrative understanding of large-scale, intact biological systems,” said senior author Dr. Karl Deisseroth, professor of bioengineering and of psychiatry and behavioral sciences at Stanford University. “It provides access to subcellular proteins and molecules, while preserving the continuity of intact neuronal structures such as long-range circuit projections, local circuit wiring, and cellular spatial relationships.”

Related Links:
Stanford University



Channels

Genomics/Proteomics

view channel
Image: Transmission electron micrograph of norovirus particles in feces (Photo courtesy of Wikimedia Commons).

Norovirus Interacts with Gut Bacteria to Establish a Persistent Infection That Can Be Blocked by Interferon Lambda

A team of molecular microbiologists and virologists has found that norovirus requires an intimate interaction with certain gut bacteria to establish a persistent infection, and that the infective process... Read more

Biochemistry

view channel

Possible New Target Found for Treating Brain Inflammation

Scientists have identified an enzyme that produces a class of inflammatory lipid molecules in the brain. Abnormally high levels of these molecules appear to cause a rare inherited eurodegenerative disorder, and that disorder now may be treatable if researchers can develop suitable drug candidates that suppress this enzyme.... Read more

Therapeutics

view channel
Image: Cancer cells infected with tumor-targeted oncolytic virus (red). Green indicates alpha-tubulin, a cell skeleton protein. Blue is DNA in the cancer cell nuclei (Photo courtesy of Dr. Rathi Gangeswaran, Bart’s Cancer Institute).

Innovative “Viro-Immunotherapy” Designed to Kill Breast Cancer Cells

A leading scientist has devised a new treatment that employs viruses to kill breast cancer cells. The research could lead to a promising “viro-immunotherapy” for patients with triple-negative breast cancer,... Read more

Business

view channel

Program Designed to Provide High-Performance Computing Cluster Systems for Bioinformatics Research

Dedicated Computing (Waukesha, WI, USA), a global technology company, reported that it will be participating in the Intel Cluster Ready program to deliver integrated high-performance computing cluster solutions to the life sciences market. Powered by Intel Xeon processors, Dedicated Computing is providing a range of... Read more
 
Copyright © 2000-2015 Globetech Media. All rights reserved.