Features | Partner Sites | Information | LinkXpress
Sign In
GLOBETECH PUBLISHING LLC
GLOBETECH PUBLISHING LLC
GLOBETECH MEDIA

Novel Method Enables Isolation of Nearly Pure Population of Mammary Gland Stem Cells

By BiotechDaily International staff writers
Posted on 25 Apr 2013
The surface protein marker CD1d is highly expressed on mammary gland stem cells (MaSCs), and the presence of this protein was used in a novel method to isolate a remarkably pure MaSC population.

CD1d is a member of the CD1 (cluster of differentiation 1) family of glycoproteins expressed on the surface of various human antigen-presenting cells. They are nonclassical major histocompatibility complex (MHC) proteins, related to the class I MHC proteins, and are involved in the presentation of lipid antigens to T-cells.

The partial purification of mouse MaSCs using a combination of cell surface markers has improved understanding of their role in normal development and breast tumorigenesis. However, despite the significant improvement in techniques for MaSC enrichment, there is presently no methodology that adequately isolates pure MaSCs.

To correct this lack, investigators at Cold Spring Harbor Laboratory (NY, USA) utilized the fact that MaSCs replicate very slowly to develop a method for their purification based on incorporation of a green fluorescent protein into the nuclei of various mammary cells including MaSCs. Since MaSCs replicate less often than other cell types, they retain more of the green fluorescent protein and can be isolated by cell sorting techniques that recognize CD1d.

The investigators reported in the April 11, 2013, online edition of the journal Proceedings of the National Academy of Sciences of the United States of America (PNAS) that working with a remarkably pure MaSC population, they were able to functionally characterize a set of MaSC-enriched genes and discovered factors controlling MaSC survival.

"With this advancement, we are now able to profile normal and cancer stem cells at a very high degree of purity, and perhaps point out which genes should be investigated as the next breast cancer drug targets," said senior author Dr. Gregory Hannon, professor of molecular biology at Cold Spring Harbor Laboratory.

Related Links:

Cold Spring Harbor Laboratory



Channels

Drug Discovery

view channel
Image: Star-like glial cells in red surround alpha-beta plaques in the cortex of a mouse with a model of Alzheimer\'s disease (Photo courtesy of Strittmatter laboratory/Yale University).

Experimental Cancer Drug Reverses Symptoms in Mouse Model of Alzheimer's Disease

An experimental, but clinically disappointing drug for treatment of cancer has been found to be extremely effective in reversing the symptoms of Alzheimer's disease (AD) in a mouse model.... Read more

Biochemistry

view channel
Image:  Model depiction of a novel cellular mechanism by which regulation of cryptochromes Cry1 and Cry2 enables coordination of a protective transcriptional response to DNA damage caused by genotoxic stress (Photo courtesy of the journal eLife, March 2015, Papp SJ, Huber AL, et al.).

Two Proteins Critical for Circadian Cycles Protect Cells from Mutations

Scientists have discovered that two proteins critical for maintaining healthy day-night cycles also have an unexpected role in DNA repair and protecting cells against genetic mutations that could lead... Read more

Business

view channel

NanoString and MD Anderson Collaborate on Development of Novel Multi-Omic Expression Profiling Assays for Cancer

The University of Texas MD Anderson Cancer Center (Houston, TX, USA) and NanoString Technologies, Inc. (Seattle, WA, USA) will partner on development of a revolutionary new type of assay—simultaneously profiling gene and protein expression, initially aiming to discover and validate biomarker signatures for immuno-oncology... Read more
 
Copyright © 2000-2015 Globetech Media. All rights reserved.