Features Partner Sites Information LinkXpress
Sign In
Demo Company

Recombinant Poultry Virus Selectively Kills Human Prostate Cancer Cells

By BiotechDaily International staff writers
Posted on 05 Mar 2013
Print article
A recombinant form of the Newcastle disease virus (NDV) that replicates in humans only in the presence of prostate specific antigen (PSA) was found to kill prostate cancer cells, including hormone resistant cells, without damaging normal tissues.

Newcastle disease is a contagious bird disease affecting many domestic and wild avian species. The virus has a negative-sense, single-stranded genome that codes for a RNA-directed RNA polymerase, hemagglutinin-neuraminidase protein, fusion (F) protein, matrix protein, phosphoprotein, and nucleoprotein in the 5' to 3' direction. Its effects are most notable in domestic poultry due to their high susceptibility and the potential for severe impacts of an epizootic on the poultry industries. Exposure of humans to infected birds can cause mild conjunctivitis and influenza-like symptoms, but NDV otherwise poses no hazard to human health. Interest in the use of NDV as an anticancer agent has arisen from the ability of NDV to selectively kill human tumor cells with limited toxicity to normal cells. However, systemically delivered NDV fails to reach solid tumors in therapeutic concentration and also spreads poorly within the tumors due to barriers including complement, innate immunity, and extracellular matrix.

Investigators at the Virginia Polytechnic Institute (Blacksburg, USA) created a novel recombinant form of NDV in which the F protein was cleavable exclusively by human PSA.

They reported in the January 23, 2013, online edition of the Journal of Virology that the recombinant NDV replicated efficiently and specifically in prostate cancer (CaP) cells and three-dimensional prostaspheres but failed to replicate in the absence of PSA. Furthermore, PSA-cleavable recombinant NDV caused specific lysis of androgen independent and androgen responsive/nonresponsive CaP cells and prostaspheres. PSA-cleavable NDV failed to replicate in chicken embryos, indicating no pathogenicity to chickens.

"Retargeted Newcastle disease virus has major potential advantages over other cancer therapies," said senior author Dr. Subbiah Elankumaran, associate professor of virology at Virginia Polytechnic Institute. "First, its specificity for prostate cancer cells means it would not attack normal cells, thereby avoiding the various unpleasant side effects of conventional chemotherapies. In previous clinical trials, even with extremely large doses of naturally occurring strains, only mild flu-like symptoms were seen in cancer patients. Second, it would provide a new treatment for hormone-refractory patients, without the side effects of testosterone suppression that result from hormonal treatments."

Related Links:
Virginia Polytechnic Institute

Print article



view channel

Molecular Light Shed on “Dark” Cellular Receptors

Scientists have created a new research tool to help find homes for orphan cell-surface receptors, toward better understanding of cell signaling, developing new therapeutics, and determining causes of drug side-effects. The approach may be broadly useful for discovering interactions of orphan receptors with endogenous, naturally... Read more

Lab Technologies

view channel
Image: The new ambr 15 fermentation micro-bioreactor system was designed to enhance microbial strain screening applications (Photo courtesy of Sartorius Stedim Biotech).

New Bioreactor System Streamlines Strain Screening and Culture

Biotechnology laboratories working with bacterial cultures will benefit from a new automated micro bioreactor system that was designed to enhance microbial strain screening processes. The Sartorius... Read more


view channel

Purchase of Biopharmaceutical Company Will Boost Development of Nitroxyl-Based Cardiovascular Disease Drugs

A major international biopharmaceutical company has announced the acquisition of a private biotech company that specializes in the development of drugs for treatment of cardiovascular disease. Bristol-Myers Squibb Co. (New York, NY, USA) has initiated the process to buy Cardioxyl Pharmaceuticals Inc. (Chapel Hill, NC, USA).... Read more
Copyright © 2000-2015 Globetech Media. All rights reserved.