Features | Partner Sites | Information | LinkXpress
Sign In
PZ HTL SA
GLOBETECH PUBLISHING LLC
GLOBETECH PUBLISHING LLC

Apolipoprotein A-IV Stabilizes Glucose Metabolism in Diabetic Mouse Model

By BiotechDaily International staff writers
Posted on 04 Jun 2012
Apolipoprotein A-IV (apoA-IV), a protein manufactured by the small intestine during the digestive process, has been found to improve the ability of diabetic mice to control their glucose levels.

Investigators at the University of Cincinnati (OH, USA) worked with cultures of isolated pancreatic cells, with mice that had been genetically engineered to lack the gene for apoA-IV, and with KKAy diabetic mice.

They reported in the May 22, 2012, online edition of the journal Proceedings of the National Academy of Sciences of the USA that apoA-IV-treated isolated pancreatic islets had enhanced insulin secretion under conditions of high glucose but not of low glucose, suggesting a direct effect of apoA-IV to enhance glucose-stimulated insulin release. This enhancement involved cAMP (3'-5'-cyclic adenosine monophosphate) at a location distant from the point entry of calcium ions into insulin-producing beta cells.

Genetically engineered “knockout” of apoA-IV resulted in compromised insulin secretion and impaired glucose tolerance compared with wild type mice. Challenging mice lacking apoAI-V with a high-fat diet led to fasting hyperglycemia and more severe glucose intolerance associated with defective insulin secretion than occurred in wild type mice. Administration of exogenous apoA-IV to the “knockout” mice improved glucose tolerance by enhancing insulin secretion in mice fed either chow or a high-fat diet.

Injection of exogenous apoA-IV decreased blood glucose levels and stimulated a transient increase in insulin secretion in KKAy diabetic mice.

Senior author Dr. Patrick Tso, professor of pathology and laboratory medicine at the University of Cincinnati, said, “ApoA-IV behaves similar to an incretin - a gastrointestinal hormone causing an increased release of insulin after eating to combat the onset of elevated blood glucose. Two well-known incretins that have been used in the development of existing diabetes medications include gastric inhibitory peptide (GIP) and glucagon-like peptide-1 (GLP-1).”

"The problem with both of these incretins is that they are short-lived - lasting only for minutes - and are quickly inactivated by an enzyme,” said Dr. Tso. “They have also been linked to hypoglycemia, or low blood sugar, when administered when the body has a low glucose concentration. The challenge is to find something safer with a longer half-life.”

With its long (seven to eight hour) half-life apoA-IV may be a suitable therapeutic target for the regulation of glucose-stimulated insulin secretion and treatment of diabetes.

Related Links:
University of Cincinnati



RANDOX LABORATORIES
BIOSIGMA S.R.L.
SLAS - Society for Laboratory Automation and Screening
comments powered by Disqus

Channels

Drug Discovery

view channel

Omega 3 Found to Improve Behavior in Children with ADHD

Supplements of the fatty acids omega 3 and 6 can help children and adolescents who have a specific kind of have attention deficit hyperactivity disorder (ADHD). Moreover, these findings indicate that a customized cognitive training program can improve problem behavior in children with ADHD. Statistics show that 3%–6%... Read more

Biochemistry

view channel

Blocking Enzyme Switch Turns Off Tumor Growth in T-Cell Acute Lymphoblastic Leukemia

Researchers recently reported that blocking the action of an enzyme “switch” needed to activate tumor growth is emerging as a practical strategy for treating T-cell acute lymphoblastic leukemia. An estimated 25% of the 500 US adolescents and young adults diagnosed yearly with this aggressive disease fail to respond to... Read more

Lab Technologies

view channel

e-Incubator Technology Provides Real-Time Imaging of Bioengineered Tissues in a Controlled Unit

A new e-incubator, an innovative miniature incubator that is compatible with magnetic resonance imaging (MRI), enables scientists to grow tissue-engineered constructs under a controlled setting and to study their growth and development in real time without risk of contamination or damage. Offering the potential to test... Read more

Business

view channel

Two Industry Partnerships Initiated to Fuel Neuroscience Research

Faster, more complex neural research is now attainable by combining technology from two research companies. Blackrock Microsystems, LLC (Salt Lake City, UT, USA), a developer of neuroscience research equipment, announced partnerships with two neuroscience research firms—PhenoSys, GmbH (Berlin, Germany) and NAN Instruments, Ltd.... Read more
 
Copyright © 2000-2014 Globetech Media. All rights reserved.