We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
LGC Clinical Diagnostics

Download Mobile App




Enzyme-Loaded Magnetic Nanoparticles Suitable for Therapeutic and Industrial Applications

By LabMedica International staff writers
Posted on 25 Apr 2016
Print article
Image: An example of magnetite with sharp, lustrous, jet-black crystals (Photo courtesy of Wikimedia Commons).
Image: An example of magnetite with sharp, lustrous, jet-black crystals (Photo courtesy of Wikimedia Commons).
Members of a novel class of nano-sized transport vessels are composed of only two biocompatible components: an enzyme and magnetite nanoparticles.

Magnetically controlled enzymatic nanoparticles have received much attention for both therapeutic and industrial applications. Such materials usually comprise at least four components: the enzyme, magnetic nanoparticles, stabilizing components, and an organic or inorganic (or hybrid) matrix as a carrier. However, such combinations can affect the magnetic response and/or the enzymatic activity, and also face regulatory restrictions that prevent them from being used for intravenous administration.

To overcome these difficulties, investigators at ITMO University (St. Petersburg, Russia) and their colleagues at the Hebrew University of Jerusalem (Israel), and Cyril and Methodius University (Skopje, Macedonia) developed a new class of magnetically controlled nanoparticles made from enzymes that have been entrapped directly within magnetite particles.

These particles were made by adding enzymes directly to a magnetite hydrosol mixture. Magnetite particles surrounded the enzymes, and after drying, formed a firm porous structure from which the enzyme could not escape.

The investigators reported in the March 16, 2016, online edition of the journal Chemistry of Materials that they had used the direct entrapment approach to create a series of magnetic biocomposites with enzymes of therapeutic and industrial interest including carbonic anhydrase, ovalbumin, horseradish peroxidase, acid phosphatase, proteinase, and xylanase.

The activity of the entrapped enzymes was studied at different temperatures and concentrations, and it was found that they showed remarkable thermal stabilization induced by the magnetite matrix. For example, entrapped carbonic anhydrase catalyzed the decomposition of p-nitrophenylacetate at a temperature of 90 degrees Celsius, while the free enzyme completely lost activity and denaturized at 70 degrees Celsius.

"The nanocomposite is absolutely biocompatible and harmless for injection into the human body," said Dr. Andrey Drozdov, a research associate in the international laboratory of solution chemistry of advanced materials and technologies at ITMO University. "Separately, both magnetite and therapeutic enzymes have medical approval for intravenous injection. Therefore, to approve their joint use should not be difficult. The body already knows what to do with these substances and how to incorporate them into the process of metabolism. Using a magnetic field, the particles can be condensed on blood clots; moreover, such systems will work for quite a long time until the enzyme is completely oxidized."

Related Links:
ITMO University
Hebrew University of Jerusalem
Cyril and Methodius University
Platinum Member
COVID-19 Rapid Test
OSOM COVID-19 Antigen Rapid Test
HLX
Complement 3 (C3) Test
GPP-100 C3 Kit
New
Gold Member
Fully Automated Cell Density/Viability Analyzer
BioProfile FAST CDV

Print article

Channels

Clinical Chemistry

view channel
Image: Reaching speeds up to 6,000 RPM, this centrifuge forms the basis for a new type of inexpensive, POC biomedical test (Photo courtesy of Duke University)

POC Biomedical Test Spins Water Droplet Using Sound Waves for Cancer Detection

Exosomes, tiny cellular bioparticles carrying a specific set of proteins, lipids, and genetic materials, play a crucial role in cell communication and hold promise for non-invasive diagnostics.... Read more

Molecular Diagnostics

view channel
Image: The study showed the blood-based cancer screening test detects 83% of people with colorectal cancer with specificity of 90% (Photo courtesy of Guardant Health)

Blood Test Shows 83% Accuracy for Detecting Colorectal Cancer

Colorectal cancer is the second biggest cause of cancer deaths among adults in the U.S., with forecasts suggesting 53,010 people might die from it in 2024. While fewer older adults are dying from this... Read more

Hematology

view channel
Image: The Gazelle Hb Variant Test (Photo courtesy of Hemex Health)

First Affordable and Rapid Test for Beta Thalassemia Demonstrates 99% Diagnostic Accuracy

Hemoglobin disorders rank as some of the most prevalent monogenic diseases globally. Among various hemoglobin disorders, beta thalassemia, a hereditary blood disorder, affects about 1.5% of the world's... Read more

Microbiology

view channel
Image: The new platform is designed to perform blood-based diagnoses of nontuberculosis mycobacteria (Photo courtesy of 123RF)

New Blood Test Cuts Diagnosis Time for Nontuberculous Mycobacteria Infections from Months to Hours

Breathing in nontuberculous mycobacteria (NTM) is a common experience for many people. These bacteria are present in water systems, soil, and dust all over the world and usually don't cause any problems.... Read more

Industry

view channel
Image: These new assays are being developed for use on the recently introduced DxI 9000 Immunoassay Analyzer (Photo courtesy of Beckman Coulter)

Beckman Coulter and Fujirebio Expand Partnership on Neurodegenerative Disease Diagnostics

Beckman Coulter Diagnostics (Brea, CA, USA) and Fujirebio Diagnostics (Tokyo, Japan) have expanded their partnership focused on the development, manufacturing and clinical adoption of neurodegenerative... Read more
Copyright © 2000-2024 Globetech Media. All rights reserved.