Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
RANDOX LABORATORIES

Events

06 Jun 2016 - 09 Jun 2016
22 Jun 2016 - 24 Jun 2016
04 Jul 2016 - 06 Jul 2016

Aspirin Found Less Effective Preventing Blood Clots in Atherosclerotic Blood Vessels

By BiotechDaily International staff writers
Posted on 02 Apr 2014
Print article
Image: Artificial blood vessels on a microfluidic chip; the researchers used a device that simulated blood flowing through narrowed coronary arteries to assess effects of anticlotting drugs (Photo courtesy of the Georgia Institute of Technology).
Image: Artificial blood vessels on a microfluidic chip; the researchers used a device that simulated blood flowing through narrowed coronary arteries to assess effects of anticlotting drugs (Photo courtesy of the Georgia Institute of Technology).
A novel microfluidic device that mimicked arterial blood flow was used to evaluate the effectiveness of anti-thrombotic drugs under various blood-flow conditions.

Investigators at the Georgia Institute of Technology (Atlanta, USA) had previously described the design and application of a microfluidic system for simultaneous optical measurement of thrombosis at multiple, well-defined shear rates simultaneously in whole pig blood without the addition of antiplatelet therapies, and presented a proof of concept for applying clinically derived pathological flow conditions to whole blood samples in a microscope-free, high throughput, microscale system.

In the current study they applied the microfluidic device to human blood across a range of shear rates and antiplatelet therapy concentrations to accurately, robustly, and statistically quantify metrics of platelet activity for a cohort of 14 human patients.

The investigators examined the effectiveness of two blood-thinning treatments: aspirin and GPIIb/IIIa-inhibitors. GPIIb/IIIa-inhibitors are generally given to patients with a higher heart attack risk. The investigators used the microfluidic device to measure occlusion times and thrombus detachment for a range of initial shear rates and therapy concentrations with eptifibatide (the GPIIb/IIIa-inhibitor), or acetyl-salicylic acid (aspirin). They also measured complete blood counts (CBC) and platelet activity using whole blood impedance aggregometry.

Results published in the January 3, 2014, online edition of the journal PLOS ONE revealed that aspirin had little effect on high shear occlusion times, even at very high doses (up to 20 times the recommended dose). Under aspirin therapy, the thrombi formed at high shear rates were four times more prone to detachment compared to those formed under control conditions. Eptifibatide reduced occlusion when controlling for shear rate, and its efficacy increased with dose concentration. In contrast, the hazard of occlusion from aspirin was several orders of magnitude higher than that of eptifibatide. The results showed similar dose efficacies to low shear measurements using whole blood aggregometry.

The investigators concluded that at lower shear rates, such as found in normal arteries, aspirin was fairly effective at stopping platelets from clumping, but at higher shear rates aspirin was not as effective at preventing these clots. The GPIIb/IIIa-inhibitor was effective at preventing blood clots across all shear rates tested, suggesting that these drugs would be effective for people with the higher shear rates caused by atherosclerosis.

“For a patient being prescribed antithrombotic drugs who is at risk for a heart attack, we can draw a small amount of their blood and quickly push a little bit through this device, and based on that information, tell them to take a certain amount of a certain drug. That is where we are going with this project,” said senior author Dr. Craig Forest, assistant professor of bioengineering at the Georgia Institute of Technology. “These microfluidic devices are so cheap and require so little blood that it could become possible for someone to use this in a disposable, rapid way.”

Related Links:

Georgia Institute of Technology



Print article

Channels

Genomics/Proteomics

view channel
Image: A dark field photomicrograph showing the spirochete bacterium Borrelia burgdorferi, the pathogen responsible for causing Lyme disease (Photo courtesy of the CDC).

Statins May Help Block Transmission of Lyme Disease

A recent study found that treatment with cholesterol-lowering statins reduced the number of Borrelia burgdorferi bacteria in rodents, which helped to block transmission of Lyme disease. Lyme disease... Read more

Biochemistry

view channel
Image: A space-filling model of the anticonvulsant drug carbamazepine (Photo courtesy of Wikimedia Commons).

Wastewater May Contaminate Crops with Potentially Dangerous Pharmaceuticals

Reclaimed wastewater used to irrigate crops is contaminated with pharmaceutical residues that can be detected in the urine of those who consumed such produce. Investigators at the Hebrew University... Read more

Lab Technologies

view channel

Huge Modifiable Biomedical Database to Be Available on the Wikidata Site

Genome researchers are exploiting the power of the open Internet community Wikipedia database to create a comprehensive resource for geneticists, molecular biologists, and other interested life scientists. While efficiency in generating scientific data improves almost daily, applying meaningful relationships between... Read more

Business

view channel

European Biotech Agreement to Promote Antigen-Drug Conjugation Technology

Two European biotech companies have joined forces to exploit and commercialize an innovative, site-specific ADC (antigen-drug conjugate) conjugation technology. ProBioGen (Berlin, Germany), a company specializing in the development and manufacture of complex glycoproteins and Eucodis Bioscience (Vienna, Austria), a... Read more
Copyright © 2000-2016 Globetech Media. All rights reserved.