Features | Partner Sites | Information | LinkXpress
Sign In
GLOBETECH PUBLISHING LLC
GLOBETECH PUBLISHING LLC
PZ HTL SA

Nonreplicating Virus Kills Leukemia in Mouse and Culture Models

By BiotechDaily International staff writers
Posted on 27 Aug 2013
Image: Electron micrograph depicts the rhabdovirus responsible for vesicular stomatitis (VS) in horses, cattle, and pigs. An inactivated rhabdovirus was used in recent studies to destroy leukemia cells (Photo couryesy of the [US] Centers for Disease Control (CDC)).
Image: Electron micrograph depicts the rhabdovirus responsible for vesicular stomatitis (VS) in horses, cattle, and pigs. An inactivated rhabdovirus was used in recent studies to destroy leukemia cells (Photo couryesy of the [US] Centers for Disease Control (CDC)).
A biopharmaceutical drug comprising nonreplicating virus particles killed leukemia cells in a mouse leukemia model and in cultures of cells taken from patients with multiple drug resistant forms of the disease.

Investigators at the University of Ottawa (Canada) speculated that high doses on a nonreplicating virus would be able to kill blood cancer cells without the dangers inherent in the use of fully competent live viruses. Toward this end they developed a method based on attenuation with UV light to produce unique high-titer bioactive yet nonreplicating rhabdovirus-derived particles (NRRPs).

This novel NRRP bio-drug was shown in a paper published in the July 12, 2013, online edition of Blood Cancer Journal to possess direct cytolytic and immunomodulatory activity against acute leukemia. In a mouse model of leukemia treated with the NRRPs 80% of the mice had markedly prolonged survival and 60% were eventually cured of the disease, while all of the untreated mice died of leukemia within 20 days.

In laboratory cultures, NRRP resistance in normal cells was mediated by intact antiviral defenses including interferon (IFN). In contrast, NRRPs administered cell cultures derived from clinical samples obtained from patients with high-burden multidrug-resistant acute myeloid leukemia killed the cancer cells.

"Our research indicated that a replicating virus might not be the safest or most effective approach for treating leukemia, so we decided to investigate whether we could make virus-derived particles that no longer replicate but still kill cancer," said senior author Dr. David Conrad, a doctor candidate in cellular and molecular medicine at the University of Ottawa. "We were delighted to see that this novel therapy was very safe at high doses, and worked extremely well in our laboratory leukemia models. We hope to test this in patients in the near future."

"Leukemia is a devastating disease that can be very difficult to treat, and new therapies are urgently needed," said Dr. Conrad. "While we are still at the early stages of this research, I think this therapy holds a lot of promise because it appears to have a potent, long-lasting effect on leukemia without the debilitating side effects of many cancer therapies used in the clinic right now. We will likely see even better results once we optimize the dose in our preparations to advance this research into human clinical trials."

Related Links:
University of Ottawa


SLAS - Society for Laboratory Automation and Screening
RANDOX LABORATORIES
BIOSIGMA S.R.L.
comments powered by Disqus

Channels

Drug Discovery

view channel

Omega 3 Found to Improve Behavior in Children with ADHD

Supplements of the fatty acids omega 3 and 6 can help children and adolescents who have a specific kind of have attention deficit hyperactivity disorder (ADHD). Moreover, these findings indicate that a customized cognitive training program can improve problem behavior in children with ADHD. Statistics show that 3%–6%... Read more

Biochemistry

view channel

Blocking Enzyme Switch Turns Off Tumor Growth in T-Cell Acute Lymphoblastic Leukemia

Researchers recently reported that blocking the action of an enzyme “switch” needed to activate tumor growth is emerging as a practical strategy for treating T-cell acute lymphoblastic leukemia. An estimated 25% of the 500 US adolescents and young adults diagnosed yearly with this aggressive disease fail to respond to... Read more

Lab Technologies

view channel
Image: Mouse kidneys, liver, and pancreas imaged after treatment with a variety of protocols: a saline solution, Scale, SeeDB (see deep brain), CUBIC, and carotid body (CB) perfusion (which was used in this study) (Photo courtesy of RIKEN Quantitative Biology Center).

Nearly Transparent Mice Offers Potential of Whole-Organism Imaging

Japanese researchers have developed a method that combines tissue decolorization and light-sheet fluorescent microscopy to take extremely detailed images of the interior of individual organs and even entire... Read more

Business

view channel

Two Industry Partnerships Initiated to Fuel Neuroscience Research

Faster, more complex neural research is now attainable by combining technology from two research companies. Blackrock Microsystems, LLC (Salt Lake City, UT, USA), a developer of neuroscience research equipment, announced partnerships with two neuroscience research firms—PhenoSys, GmbH (Berlin, Germany) and NAN Instruments, Ltd.... Read more
 
Copyright © 2000-2014 Globetech Media. All rights reserved.