Features | Partner Sites | Information | LinkXpress
Sign In
GLOBETECH PUBLISHING
JIB
BioConferenceLive

Genetically Engineered Mouse Models That Closely Resemble Human Patients Enable More Relevant Cancer Drug Studies

By BiotechDaily International staff writers
Posted on 18 Jul 2013
Image: Ultrasound imaging of mouse tumor showing response to chemotherapy. The mouse model allowed researchers to derive a new biomarker of chemotherapy responsiveness (Photo courtesy of Perou Laboratory at the University of North Carolina).
Image: Ultrasound imaging of mouse tumor showing response to chemotherapy. The mouse model allowed researchers to derive a new biomarker of chemotherapy responsiveness (Photo courtesy of Perou Laboratory at the University of North Carolina).
The use of genetically engineered mouse models (GEMMs) as recipients for xenografts of various types of human tumors enables study of tumor growth in an animal system with intact immune system and identification of genetic signatures that can be used to predict the responsiveness of these tumors to drug treatment.

Investigators at the University of North Carolina (Chapel Hill, USA) reasoned that drug studies conducted using traditional mouse models, which lack functional immune systems, could produce misleading results. To evaluate this theory they examined the efficacy of four chemotherapeutic or targeted anticancer drugs, alone and in combination, using mouse models representing three distinct breast cancer subtypes: Basal-like (GEMM), Luminal B (GEMM), and Claudin-low (non-GEMM). Drugs tested as single agents included carboplatin, paclitaxel, erlotinib, and lapatinib. The investigators used RNA expression analysis to profile tumors in order to develop signatures that corresponded to treatment and response and then tested their predictive potential using human patient data.

Results published in the June 18, 2013, online edition of the journal Clinical Cancer Research revealed that while lapatinib alone exhibited exceptional efficacy in one model system, generally single-agent activity was modest, while some combination therapies were more active and life prolonging. Through analysis of RNA expression in this large set of chemotherapy-treated mouse tumors, a pair of gene expression signatures was identified that predicted pathological complete response to neoadjuvant anthracycline/taxane (doxorubicin/paclitaxel) therapy in human patients with breast cancer.

Results presented in this study showed that mouse-derived gene signatures could predict drug response even after accounting for common clinical variables and other predictive genomic signatures, suggesting that mice could be used to identify new biomarkers for human cancer patients. Senior author Dr. Charles Perou, professor of molecular oncology research at the University of North Carolina, said, “This is a wonderful example of how well chosen mouse models can inform a human disease state. In this case we used years of research to match the models to specific human subtypes, and then treated the animals with therapies identical to what human cancer patients are receiving. We were ultimately able to develop a biomarker of treatment response from the mouse that works in humans.”

Related Links:
University of North Carolina



comments powered by Disqus

Channels

Genomics/Proteomics

view channel
Image: Scanning electron micrograph of cultured human neuron from induced pluripotent stem cell (Photo courtesy of the University of California, San Diego).

Induced Stem Cells Show Genetic Abnormalities Not Found in Embryonic Stem Cells

All stem cells are not created equal: genomic evaluation revealed that the genome of stem cells generated from adult cells (induced pluripotent stem cells or iPS cells) differed considerably from that... Read more

Drug Discovery

view channel
Image: A mouse mammary gland missing the tumor-suppressor p53 shows expression of ARF (green), now known for a backup role in protecting cells from becoming cancerous. If both p53 and ARF are mutated, the tumors that form are aggressive and may benefit from treatment with anti-inflammatory drugs called JAK inhibitors, currently prescribed for rheumatoid arthritis (Photo courtesy of Raleigh Kladney).

Anti-Inflammatory Drugs May Treat Some Aggressive Rumors

New research raises the possibility that some cancer patients with aggressive tumors may benefit from a class of anti-inflammatory drugs used to treat rheumatoid arthritis. By studying triple-negative... Read more

Therapeutics

view channel

Vaccine Being Developed for Heart Disease Close to Reality

The world’s first vaccine for heart disease is becoming a possibility with researchers demonstrating significant arterial plaque reduction in concept testing in mice. Klaus Ley, MD, from the La Jolla Institute for Allergy and Immunology (LA Jolla, CA, USA), and a vascular immunology specialist, is leading the vaccine... Read more

Business

view channel

A Surge in IPOs Revitalize Investments for the Global Pharma and Biotech

Anti-infective drugs, oncology, and pharmaceutical contract laboratories attract the most investment up to now. The intensified private equity and venture capital (PEVC) deal activity in the global healthcare industry during the recession years, 2008–2010, witnessed a waning post-2010. However, the decline in deals... Read more
 
Copyright © 2000-2014 Globetech Media. All rights reserved.