Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
RANDOX LABORATORIES

Events

10 May 2016 - 16 May 2016
11 May 2016 - 13 May 2016

Treatment with Fleeceflower Derivative Blocks Plaque Formation in Mouse Alzheimer's Disease Model

By BiotechDaily International staff writers
Posted on 26 Feb 2013
Print article
A compound derived from traditional Chinese herbal medicine prevented development of amyloid plaques when administered to young animals in a mouse model of Alzheimer's disease and reversed plaque formation when administered to older animals.

Investigators at the Capital Medical University (Beijing, China) worked with the APPV717I transgenic (Tg) mouse Alzheimer's disease model. In previous studies, these mice demonstrated cognitive impairments beginning at four months of age and developed amyloid plaques in the brain that were evident by 10 months.

In the current study Tg mice were treated with varying doses of tetrahydroxystilbene glucoside (TSG), the active compound derived from the Chinese herbal medicine tuber fleeceflower (Polygonum multiflorum Thunb). The drug was given to 4- or 10- month-old animals for a period of six months. At the end of the treatment, cDNA microarray analysis, reverse transcription PCR, western blotting, and immunochemical techniques were used to determine levels of alpha-synuclein messenger RNA (mRNA) and protein expression. Accumulation of alpha-synuclein leads to the formation of insoluble aggregates that have been implicated in several neurodegenerative diseases, including Parkinson's disease, dementia with Lewy bodies, and Alzheimer's disease.

Results published in the January 2013 issue of the journal Restorative Neurology and Neuroscience revealed that alpha-synuclein mRNA expression increased in the hippocampus of four-month to 16-month old Tg mice compared with age-matched non-Tg controls. Alpha-synuclein protein expression in the hippocampus also increased significantly in four-month to 16-month old Tg mice. Significant down-regulation of alpha-synuclein mRNA and protein expression in the hippocampus was found after treatment with TSG for six months in both 10- and 16-month-old Tg mice. Production of dimers and tetramers of alpha-synuclein protein in Tg mice was inhibited after treatment with TSG.

The expression and aggregation of alpha-synuclein increased in an age-dependent fashion in Tg mice. TSG not only prevented over-expression of alpha-synuclein at an early stage, but also reversed the increased expression of alpha-synuclein and inhibited aggregation at the late stage of Tg mice. Therefore, TSG may have potential to prevent and treat Alzheimer's disease.

"Our results raise the possibility that TSG might be a novel compound for the treatment of Alzheimer's disease and dementia with Lewy body," said first author Dr. Lan Zhang, associate professor of pharmacology at Capital Medical University. "The role of alpha-synuclein, especially in the early phase of Alzheimer's disease, and its interaction with amyloid-beta should be considered when developing new therapeutic strategies to target Alzheimer's disease."

Related Links:

Capital Medical University



Print article

Channels

Genomics/Proteomics

view channel
Image: An expression of NOTCH 1 (green color) in ACC stem cells (Photo courtesy of Yale University).

Adenoid Cystic Carcinoma Stem Cells Depend on NOTCH1 and SOX10 Signaling

Cancer researchers have isolated a stem cell population from the cells making up an adenoid cystic carcinoma (ACC) tumor and showed that the signaling factors NOTCH1 and SOX10 were essential for the cancer... Read more

Biochemistry

view channel
Image: A space-filling model of the anticonvulsant drug carbamazepine (Photo courtesy of Wikimedia Commons).

Wastewater May Contaminate Crops with Potentially Dangerous Pharmaceuticals

Reclaimed wastewater used to irrigate crops is contaminated with pharmaceutical residues that can be detected in the urine of those who consumed such produce. Investigators at the Hebrew University... Read more

Lab Technologies

view channel

Huge Modifiable Biomedical Database to Be Available on the Wikidata Site

Genome researchers are exploiting the power of the open Internet community Wikipedia database to create a comprehensive resource for geneticists, molecular biologists, and other interested life scientists. While efficiency in generating scientific data improves almost daily, applying meaningful relationships between... Read more

Business

view channel

European Biotech Agreement to Promote Antigen-Drug Conjugation Technology

Two European biotech companies have joined forces to exploit and commercialize an innovative, site-specific ADC (antigen-drug conjugate) conjugation technology. ProBioGen (Berlin, Germany), a company specializing in the development and manufacture of complex glycoproteins and Eucodis Bioscience (Vienna, Austria), a... Read more
Copyright © 2000-2016 Globetech Media. All rights reserved.