Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
Micromedic Technologies

New Technique Developed for Species-Level Identification of Live Gram-Negative Bacteria

By BiotechDaily International staff writers
Posted on 04 Feb 2014
Print article
A new method has now been developed that specifically detects and identifies live Legionella pneumophila in aqueous samples much more quickly than conventional methods, enabling more effective monitoring to prevent epidemics.

Legionella pneumophila proliferates in systems of standing water between 25–50 °C, such as in fountains, boilers, reservoirs, whirlpool tubs, and intermittently used water pipes. Although drinking the contaminated water poses no risk in itself, inhaling droplets often leads to severe lung infections, with a relatively high fatality rate. Frequent monitoring of water samples is necessary to prevent epidemics, but the traditional culture-based method takes several days.

Prof. Sam Dukan, Prof. Boris Vauzeilles, and their team at the multiple institutes of The National Centre for Scientific Research, France (CNRS) developed the new method that specifically identifies live Legionella pneumophila within 1 day. As a gram-negative bacterium, the Legionella cell wall is made up of a species-specific lipopolysaccharide. For the new test, samples are exposed to an azide(N3)-modified precursor compound that only Legionella pneumophila specifically incorporates into its cell wall saccharide units. The azide groups can then be used to bind various (e.g., fluorescent marker) detection probes to the cell surface for detection and identification of the pathogen.

This new technique, described by Mas Pons J., et al. in the journal Angewandte Chemie (International Edition), January 27, 2014, provides easy, relatively rapid, selective detection of a single species of live bacteria, and is the first reported metabolic lipopolysaccharide labeling using a species-specific saccharide for this purpose.

Related Links:

CNRS - Centre national de la recherche scientifique



Print article

Channels

Genomics/Proteomics

view channel
Image: Top: Overview of the structure of T-STAR STAR domain in complex with AUUAAA RNA. Bottom left: close up view of the specific recognition of the RNA. Bottom right: close up view of the KH dimerization interface (Photo courtesy of the University of Leicester).

Atomic Structures of Alternative Splicing Regulator Proteins May Lead to New Anticancer Drugs

A detailed structural analysis of two RNA-binding proteins that regulate alternate splicing of gene expression, which can lead to various types of cancer, is expected to aid in the development of drugs... Read more

Drug Discovery

view channel
Image: Naturally occurring clay from Kisameet Bay, Canada, exhibits potent antibacterial activity against multidrug-resistant pathogens (Photo courtesy of Kisameet Glacial Clay Inc.).

Antibiotic Resistant Bacteria Succumb to Treatment with Unique Natural Clay

A team of Canadian medical microbiologists has demonstrated the potential use of a unique type of natural clay for treating pathogenic bacteria that have become resistant to the commonly used antibiotics.... Read more

Business

view channel

Purchase Agreement to Boost Ebola Vaccine Development

A deal to help boost development of a vaccine to protect against Ebolavirus infection was finalized at the recent Davos Conference in Switzerland. Gavi (Geneva, Switzerland), the global alliance for vaccines and immunizations, announced that it would spend five million USD to purchase the Ebola vaccine under development... Read more
Copyright © 2000-2016 Globetech Media. All rights reserved.