Features | Partner Sites | Information | LinkXpress
Sign In
GLOBETECH MEDIA
GLOBETECH PUBLISHING LLC
GLOBETECH PUBLISHING LLC

New Technique Developed for Species-Level Identification of Live Gram-Negative Bacteria

By BiotechDaily International staff writers
Posted on 04 Feb 2014
A new method has now been developed that specifically detects and identifies live Legionella pneumophila in aqueous samples much more quickly than conventional methods, enabling more effective monitoring to prevent epidemics.

Legionella pneumophila proliferates in systems of standing water between 25–50 °C, such as in fountains, boilers, reservoirs, whirlpool tubs, and intermittently used water pipes. Although drinking the contaminated water poses no risk in itself, inhaling droplets often leads to severe lung infections, with a relatively high fatality rate. Frequent monitoring of water samples is necessary to prevent epidemics, but the traditional culture-based method takes several days.

Prof. Sam Dukan, Prof. Boris Vauzeilles, and their team at the multiple institutes of The National Centre for Scientific Research, France (CNRS) developed the new method that specifically identifies live Legionella pneumophila within 1 day. As a gram-negative bacterium, the Legionella cell wall is made up of a species-specific lipopolysaccharide. For the new test, samples are exposed to an azide(N3)-modified precursor compound that only Legionella pneumophila specifically incorporates into its cell wall saccharide units. The azide groups can then be used to bind various (e.g., fluorescent marker) detection probes to the cell surface for detection and identification of the pathogen.

This new technique, described by Mas Pons J., et al. in the journal Angewandte Chemie (International Edition), January 27, 2014, provides easy, relatively rapid, selective detection of a single species of live bacteria, and is the first reported metabolic lipopolysaccharide labeling using a species-specific saccharide for this purpose.

Related Links:

CNRS - Centre national de la recherche scientifique



WATERS CORPORATION

Channels

Drug Discovery

view channel
Image: Molecular model of the anti-cancer drug 5-fluorouracil (Photo courtesy of Wikimedia Commons).

Novel Microcapsule Approach Reduces Toxic Side Effects of Chemotherapy

Cancer researchers have reduced chemotherapy's toxic side effects by using nanoporous capsules to transport an enzyme to the site of a tumor where it is activated by a selective heating process to convert... Read more

Lab Technologies

view channel
Image: The gene assembly robot, the GeneTheatre (Photo courtesy of Analytik Jena AG).

Genomic Research Laboratories Await New Compact Liquid Handling System

A small footprint benchtop liquid handler that automates multiple gene assembly tasks and associated procedures such as PCR setup is now available for use by biotech and genomic research laboratories.... Read more

Business

view channel

NanoString and MD Anderson Collaborate on Development of Novel Multi-Omic Expression Profiling Assays for Cancer

The University of Texas MD Anderson Cancer Center (Houston, TX, USA) and NanoString Technologies, Inc. (Seattle, WA, USA) will partner on development of a revolutionary new type of assay—simultaneously profiling gene and protein expression, initially aiming to discover and validate biomarker signatures for immuno-oncology... Read more
 

Events

27 May 2015 - 28 May 2015
02 Jun 2015 - 03 Jun 2015
15 Jun 2015 - 18 Jun 2015
Copyright © 2000-2015 Globetech Media. All rights reserved.