Features | Partner Sites | Information | LinkXpress
Sign In
PZ HTL SA
GLOBETECH PUBLISHING LLC
GLOBETECH PUBLISHING LLC

New Technique Developed for Species-Level Identification of Live Gram-Negative Bacteria

By BiotechDaily International staff writers
Posted on 04 Feb 2014
A new method has now been developed that specifically detects and identifies live Legionella pneumophila in aqueous samples much more quickly than conventional methods, enabling more effective monitoring to prevent epidemics.

Legionella pneumophila proliferates in systems of standing water between 25–50 °C, such as in fountains, boilers, reservoirs, whirlpool tubs, and intermittently used water pipes. Although drinking the contaminated water poses no risk in itself, inhaling droplets often leads to severe lung infections, with a relatively high fatality rate. Frequent monitoring of water samples is necessary to prevent epidemics, but the traditional culture-based method takes several days.

Prof. Sam Dukan, Prof. Boris Vauzeilles, and their team at the multiple institutes of The National Centre for Scientific Research, France (CNRS) developed the new method that specifically identifies live Legionella pneumophila within 1 day. As a gram-negative bacterium, the Legionella cell wall is made up of a species-specific lipopolysaccharide. For the new test, samples are exposed to an azide(N3)-modified precursor compound that only Legionella pneumophila specifically incorporates into its cell wall saccharide units. The azide groups can then be used to bind various (e.g., fluorescent marker) detection probes to the cell surface for detection and identification of the pathogen.

This new technique, described by Mas Pons J., et al. in the journal Angewandte Chemie (International Edition), January 27, 2014, provides easy, relatively rapid, selective detection of a single species of live bacteria, and is the first reported metabolic lipopolysaccharide labeling using a species-specific saccharide for this purpose.

Related Links:

CNRS - Centre national de la recherche scientifique



comments powered by Disqus

Channels

Genomics/Proteomics

view channel

New Program Encourages Wide Distribution of Genomic Data

A new data sharing program allows genomics researchers and practitioners to analyze, visualize, and share raw sequence data for individual patients or across populations straight from a local browser. The sequencing revolution is providing the raw data required to identify the genetic variants underlying rare diseases... Read more

Drug Discovery

view channel
Image: The nano-cocoon drug delivery system is biocompatible, specifically targets cancer cells, can carry a large drug load, and releases the drugs very quickly once inside the cancer cell. Ligands on the surface of the \"cocoon\" trick cancer cells into consuming it. Enzymes (the “worms\" in this image) inside the cocoon are unleashed once inside the cell, destroying the cocoon and releasing anticancer drugs into the cell (Photo courtesy of Dr. Zhen Gu, North Carolina State University).

Novel Anticancer Drug Delivery System Utilizes DNA-Based Nanocapsules

A novel DNA-based drug delivery system minimizes damage to normal tissues by utilizing the acidic microenvironment inside cancer cells to trigger the directed release of the anticancer drug doxorubicin (DOX).... Read more

Lab Technologies

view channel

Experimental Physicists Find Clues into How Radiotherapy Kills Cancer Cells

A new discovery in experimental physics has implications for a better determination of the process in which radiotherapy destroys cancer cells. Dr. Jason Greenwood from Queen’s University Belfast (Ireland) Center for Plasma Physics collaborated with scientists from Italy and Spain on the work on electrons, and published... Read more

Business

view channel

Interest in Commercial Applications for Proteomics Continues to Grow

Increasing interest in the field of proteomics has led to a series of agreements between private proteomic companies and academic institutions as well as deals between pharmaceutical companies and novel proteomics innovator biotech companies. Proteomics is the study of the structure and function of proteins.... Read more
 
Copyright © 2000-2014 Globetech Media. All rights reserved.