Features | Partner Sites | Information | LinkXpress
Sign In
GLOBETECH PUBLISHING LLC
GLOBETECH MEDIA
GLOBETECH PUBLISHING LLC

New Technique Developed for Species-Level Identification of Live Gram-Negative Bacteria

By BiotechDaily International staff writers
Posted on 04 Feb 2014
A new method has now been developed that specifically detects and identifies live Legionella pneumophila in aqueous samples much more quickly than conventional methods, enabling more effective monitoring to prevent epidemics.

Legionella pneumophila proliferates in systems of standing water between 25–50 °C, such as in fountains, boilers, reservoirs, whirlpool tubs, and intermittently used water pipes. Although drinking the contaminated water poses no risk in itself, inhaling droplets often leads to severe lung infections, with a relatively high fatality rate. Frequent monitoring of water samples is necessary to prevent epidemics, but the traditional culture-based method takes several days.

Prof. Sam Dukan, Prof. Boris Vauzeilles, and their team at the multiple institutes of The National Centre for Scientific Research, France (CNRS) developed the new method that specifically identifies live Legionella pneumophila within 1 day. As a gram-negative bacterium, the Legionella cell wall is made up of a species-specific lipopolysaccharide. For the new test, samples are exposed to an azide(N3)-modified precursor compound that only Legionella pneumophila specifically incorporates into its cell wall saccharide units. The azide groups can then be used to bind various (e.g., fluorescent marker) detection probes to the cell surface for detection and identification of the pathogen.

This new technique, described by Mas Pons J., et al. in the journal Angewandte Chemie (International Edition), January 27, 2014, provides easy, relatively rapid, selective detection of a single species of live bacteria, and is the first reported metabolic lipopolysaccharide labeling using a species-specific saccharide for this purpose.

Related Links:

CNRS - Centre national de la recherche scientifique



Channels

Genomics/Proteomics

view channel
Image: Typical antibodies (left) unfold in the harsh environment of the cell. Camelid antibodies (right) are smaller and more stable (Photo courtesy of the Washington University School of Medicine).

Double Targeting Approach Increases Potential for Cancer Treatment with Oncolytic Viruses

Cancer researchers have used a double targeting approach to direct oncolytic viruses specifically to tumor cells where they reproduce until the cancer cells burst, releasing more viruses to infect and... Read more

Drug Discovery

view channel
Image: Molecular model of the protein Saposin C (Photo courtesy of Wikimedia Commons).

Nanovesicles Kill Human Lung Cancer Cells in Culture and in a Mouse Xenograft Model

Nanovesicles assembled from the protein Saposin C (SapC) and the phospholipid dioleoylphosphatidylserine (DOPS) were shown to be potent inhibitors of lung cancer cells in culture and in a mouse xenograft model.... Read more

Biochemistry

view channel

Possible New Target Found for Treating Brain Inflammation

Scientists have identified an enzyme that produces a class of inflammatory lipid molecules in the brain. Abnormally high levels of these molecules appear to cause a rare inherited eurodegenerative disorder, and that disorder now may be treatable if researchers can develop suitable drug candidates that suppress this enzyme.... Read more

Lab Technologies

view channel
Image: The FLUOVIEW FVMPE-RS Gantry microscope (Photo courtesy of Olympus).

New Multiphoton Laser Scanning Microscope Configurations Expand Research Potential

Two new configurations of a state-of-the-art multiphoton laser scanning microscope extend the usefulness of the instrument for examining rapidly occurring biological events and for obtaining images from... Read more

Business

view channel

Roche Acquires Signature Diagnostics to Advance Translational Research

Roche (Basel, Switzerland) will advance translational research for next generation sequencing (NGS) diagnostics by leveraging the unique expertise of Signature Diagnostics AG (Potsdam, Germany) in biobanks and development of novel NGS diagnostic assays. Signature Diagnostics is a privately held translational oncology... Read more
 
Copyright © 2000-2015 Globetech Media. All rights reserved.