Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
RANDOX LABORATORIES

Events

10 Oct 2016 - 12 Oct 2016
12 Nov 2016 - 16 Nov 2016

Novel Technique Improves Investigation of Interactions Between HIV and Malaria Parasite

By BiotechDaily International staff writers
Posted on 27 Aug 2012
Print article
A new video article describes a novel technique used to study the interactions between HIV-1 and Plasmodium falciparum in cultured human cells, allowing scientists to explore different parameters of coinfection by the two microbes.

Due to their extensive overlap in some developing regions, especially Sub-Saharan Africa, coinfections with malaria and HIV-1 are common, but the interplay between the two diseases is poorly understood and a systematic analysis of the interactions in different relevant human primary cell populations is critically needed. Each disease affects the immune system differently and by studying coinfection at different phases of each disease in vitro, scientists can better understand how different stages of malaria infection and HIV reproduction affect the onset and severity of the other disease.

In this study, an in vitro Plasmodium-HIV-1 coinfection model was developed and used to investigate the impact of P. falciparum-infected red blood cells on the HIV-1 replicative cycle in human primary monocyte-derived macrophages (MDMs). The impact of parasite exposure on HIV-1 transcriptional/translational events was monitored by using single cycle pseudotyped viruses in which a luciferase reporter gene has replaced the Env gene, while the effect on the quantity of progeny virus released by the infected macrophages is determined by measuring the HIV-1 capsid protein p24 by ELISA in cell supernatants. The researchers observed that exposure of P. falciparum to MDMs, decreases their susceptibility to HIV-1 infection, exerting a clear detrimental effect on the HIV-1 replicative cycle in macrophages (not excluding the possibility of other effects under different conditions).

The new technique, developed and applied by the laboratory led by Dr. David Richard of the Centre Hospitalier Universitaire de Quebec (CHUQ; Quebec City, Quebec, CA; www.chuq.qc.ca), was published August 15, 2012, in the online video Journal of Visualized Experiments (JoVE). "By publishing in JoVE, you really see what is happening in the experiment. The visual representation helps succinctly explain a long procedure, and gives you a fuller picture of the schematic complexity," said Dr. Richards. He hopes that this publication will give the scientific community an important additional tool to look at the interactions of the coinfection encounter on a cellular level and to more thoroughly dissect these interactions in a simplified system. This versatile system can also be adapted to monitor other factors and to use other primary cell types susceptible to HIV-1 infection. "Publication of the protocol in JoVE will allow researchers around the world to see a detailed demonstration of this system, which will help bring the technology to their laboratories," said JoVE editor Dr. Charlotte Frank Sage.

Related Links:

Centre Hospitalier Universitaire de Quebec
Journal of Visualized Experiments




Print article

Channels

Drug Discovery

view channel
Image: Ginger is the source of a novel class of nanolipid transport vector (Photo courtesy of Georgia State University).

Ginger-Derived Doxorubicin-Loaded Nanovectors as Drug Delivery for Cancer Therapy

A novel type of nanoparticle drug transport system based on lipids isolated from ginger was used to deliver the toxic chemotherapeutic agent doxorubicin (Dox) to colon cancer cells with minimal damage... Read more

Biochemistry

view channel
Image: A space-filling model of the anticonvulsant drug carbamazepine (Photo courtesy of Wikimedia Commons).

Wastewater May Contaminate Crops with Potentially Dangerous Pharmaceuticals

Reclaimed wastewater used to irrigate crops is contaminated with pharmaceutical residues that can be detected in the urine of those who consumed such produce. Investigators at the Hebrew University... Read more

Business

view channel

Collaborative Agreement to Aid in Setting Guidelines for Evaluating Potential Ebola Therapy

Cooperation between an Israeli biopharmaceutical company and medical branches of the US government is designed to set ground rules for continued evaluation of an experimental therapy for Ebola virus disease. RedHill Biopharma Ltd. (Tel Aviv, Israel), a biopharmaceutical company primarily focused on development and c... Read more
Copyright © 2000-2016 Globetech Media. All rights reserved.